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EMAGE:5911

Fgf13 fibroblast growth factor 13 ( MGI:109178)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5911
Figure 4J. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.mod.2008.04.006] Mech Dev 125: 687-99, Heanue TA; Pachnis V, "Ret isoform function and marker gene expression in the enteric nervous system is conserved across diverse vertebrate species."Copyright 2008. [PMID:18565740]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: arrowhead - domain of expression other than enteric precursors; area bounded by hatch marks - developing foregut.
Expression Pattern Description
Spatial Annotation:
EMAGE:5911Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5911_wholemount__moderate.wlz
5911_wholemount__possible.wlz
5911_wholemount__notDetected.wlz
5911_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5911_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
nervous system
detected detected
regionalThere is no expression in the enteric precursors colonizing the developing foregut but there is expression in some neural domains outwith the enteric nervous system.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3814902
Entity Detected:Fgf13, fibroblast growth factor 13 ( MGI:109178)
Sequence:sense strand is shown

>MGI:3814902
GCCTGCCTCCTGCTAGTCTCGCCTGCCATCCCGGCCCGGGGCTGTGGCGTCGGTTCGGACCAAGGCTACC
AGAACCCGCGCGGGAGCCCGAGCACCTTCGGAGGAGCTCAGAAGGCATGCTGAGCCCCCTCCGCGACTGA
AGCCGGAGTGCCCAGAAGCCCCGGCCAAGCAAGCGCAGGCTAAGGAGACCCAGGCGGCCAAGGCGCGAGC
AGCGCAGCAGCGGAGGAGCGAGAGGAGGCGAAGGCAGAGGGGCCCCCGAAGAAGTGGTGGTGGTGGGCGT
CGTGGCCATGGCGGCGGCTATCGCCAGCTCGCTCATCCGGCAAAAGAGACAAGCCCGCGAGCGCGAGAAA
TCCAATGCCTGCAAGTGTGTCAGCAGCCCCAGCAAAGGCAAGACCAGCTGCGACAAAAACAAGTTAAACG
TCTTTTCCCGGGTCAAACTCTTTGGCTCCAAGAAGAGGCGCAGAAGGAGACCAGAGCCTCAGCTTAAGGG
TATAGTTACCAAACTATACAGCCGACAAGGCTACCACTTGCAACTGCAGGCAGATGGAACCATTGATGGC
ACCAAAGACGAGGACAGCACTTACACTCTGTTTAACCTCATCCCTGTGGGTCTTCGGGTGGTGGCTATTC
AAGGAGTTCAAACCAAGCTGTATTTGGCAATGAACAGCGAGGGATACTTGTACACCTCGGAACATTTCAC
ACCTGAGTGCAAATTCAAAGAATCAGTGTTTGAAAATTATTACGTGACATACTCATCAATGATCTACCGT
CAGCAGCAATCCGGCCGAGGGTGGTATCTAGGTCTGAACAAAGAAGGAGAGATCATGAAAGGCAACCATG
TGAAGAAGAACAAGCCTGCAGCACATTTTCTGCCCAAACCACT
nt 87 - nt 969 of NM_010200.2
Notes:The Fgf13 probe used in this study by Heanue & Pachnis, 2008 [PMID:18565740] was "generated by RT-PCR (Fwd - GCCTGCCTCCTGCTAGTCT, Rev - AGTGGTTTGGGCAGAAAATG) cloned into pGEM-T Easy, clone named Fgf13-2-2, digest with SacII, use SP6 polymerase".
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Heanue TA & Pachnis V, 2008 [PMID:18565740] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.mod.2008.04.006] [ PMID:18565740] Heanue TA, Pachnis V 2008 Ret isoform function and marker gene expression in the enteric nervous system is conserved across diverse vertebrate species. Mech Dev (125):687-99
Links:MGI:3814913 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI