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EMAGE:5914

Ret ret proto-oncogene ( MGI:97902)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5914
Figure 4A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.mod.2008.04.006] Mech Dev 125: 687-99, Heanue TA; Pachnis V, "Ret isoform function and marker gene expression in the enteric nervous system is conserved across diverse vertebrate species."Copyright 2008. [PMID:18565740]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: arrowhead - expression in a population of enteric precursors invading the foregut; area bounded by hatch marks - developing foregut.
Expression Pattern Description
Spatial Annotation:
EMAGE:5914Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5914_wholemount__moderate.wlz
5914_wholemount__weak.wlz
5914_wholemount__notDetected.wlz
5914_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5914_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
peripheral nervous system
detected detected
regionalRet is expressed in enteric precursors colonizing the developing foregut
foregut
detected detected
Ret is expressed in enteric precursors colonizing the developing foregut
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:17850
Entity Detected:Ret, ret proto-oncogene ( MGI:97902)
Notes:The Ret probe used in this study by Heanue & Pachnis, 2008 [PMID:18565740] , is indicated as that described by Heanue & Pachnis, 2006 [PMID:16632597] i.e. generated from the Pachnis lab stock clone (pmcRet7), linearised with NotI and transcribed with T7 polymerase. Editors note: The pmcRet7 clone was first described by Pachnis et al, 1993 [PMID:8306871] - see Fig1A for a schematic representation. It covers the 3' portion of the coding region (a small part of the extracellular domain, the transmembrane and intracellular domains) and the 3' untranslated region.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Heanue TA & Pachnis V, 2008 [PMID:18565740] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.mod.2008.04.006] [ PMID:18565740] Heanue TA, Pachnis V 2008 Ret isoform function and marker gene expression in the enteric nervous system is conserved across diverse vertebrate species. Mech Dev (125):687-99
 [ PMID:8306871] Pachnis V, Mankoo B, Costantini F 1993 Expression of the c-ret proto-oncogene during mouse embryogenesis. Development (119):1005-17
 [ doi:10.1073/pnas.0602152103] [ PMID:16632597] Heanue TA, Pachnis V 2006 Expression profiling the developing mammalian enteric nervous system identifies marker and candidate Hirschsprung disease genes. Proc Natl Acad Sci U S A (103):6919-24
Links:MGI:3814910 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI