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EMAGE:5928

Hnf1b HNF1 homeobox B ( MGI:98505)
TS15 (9.5 dpc)
in situ reporter

Data Images
EMAGE:5928 EMAGE:5928
Figure 2J. Copyright: This image is from Barbacci E, Development 1999 Nov;126(21):4795-805 and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. [PMID:10518496] Figure 2K. Copyright: This image is from Barbacci E, Development 1999 Nov;126(21):4795-805 and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. [PMID:10518496]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: ov - otic vesicle, nt - neural tube, pb - pancreatic bud, g - gut, m - mesonephros, roof plate, sg - spinal ganglia. K shows a cross section through the neural tube in dark field (where LacZ stain appears red/pink).
Expression Pattern Description
Spatial Annotation:
EMAGE:5928Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5928_wholemount__moderate.wlz
5928_wholemount__weak.wlz
5928_wholemount__notDetected.wlz
5928_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5928_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
neural tube
detected detected
regionalExpression in the neural tube extended from the posterior border of the otic vesicle to the forelimb bud level(Fig 2J). Expression is predominant in the ventral part and in the roof plate (Fig 2K).
foregut
detected detected
regionalExpression was detected in the dorsal foregut.
mesonephros
detected detected
foregut-midgut junction
detected detected
regionalExpressed in pancreas primordium
future hindbrain
detected detected
regionalExpression in the neural tube extended from the posterior border of the otic vesicle to the forelimb bud level.
spinal ganglion
detected detected
neural tube roof plate
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ reporter
Entity Detected:Hnf1b, HNF1 homeobox B ( MGI:98505)
Notes:Hnf1b expression was revealed in this study by Barbacci et al., 1999 [PMID:10518496] in embryos heterozygous for a targeted allele where exon 1 was replaced by a construct containing lacZ and neo. The deleted coding sequence encodes the entire N-terminal dimerisation domain and a portion of another domain showing homology to either the human POU-A subdomain or human B-domain.
Labelled with:LacZ
Specimen
Organism:mouse
Strain:either: (involves: 129P2/OlaHsd) or (involves: 129P2/OlaHsd * C57BL/6J) or (involves: 129P2/OlaHsd * C57BL/6J * DBA/2)
Age:9.5 dpc
Theiler Stage:TS15
Mutations:Hnf1btm1Sce/Hnf1b+
Preparation:wholemount
Procedures
Fixation:0.2% glutaraldehyde, 2% formaldehyde, 2mM MgCl2, 5mM EGTA
General Information
Authors:Barbacci E; Reber M; Ott MO; Breillat C; Huetz F; Cereghini S, 1999 [PMID:10518496] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10518496] Barbacci E, Reber M, Ott MO, Breillat C, Huetz F, Cereghini S 1999 Variant hepatocyte nuclear factor 1 is required for visceral endoderm specification. Development (126):4795-805
Links:MGI:3798190 same experiment
 MGI:1857856 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI