Type: | in situ hybridisation probe |
Identifier: | MGI:3789342 |
Entity Detected: | Lingo2, leucine rich repeat and Ig domain containing 2 ( MGI:2442298) |
Sequence: | sense strand is shown
>MGI:3789342
GGACGTGAGGTTGAGACCATAGAGGCTGTTGGCTGGCATCAAATCCAACAAAGGCCAATAGTCGATCTCT
AGGTTTTTCAGGTGGAACAATCTTTTAAAGGCATACACAGGCATATTGTTGATATTGAGATGCTTCAGAT
GCAGGGCGATGAGGCTGCGGAGATGGGAAAGGGCTTCTGTTGGTACTGCTGTCAAGTTGCACTTCTCCAG
GGTGAGCTGCTCCAAGCTAAGTAGTCCGCTGAAGGCCCTGTGTGAGATATACACTAAATCATTGTCCCCC
ACTTCTAGAGACTTCAGGTTATGCAGATCCTGGAACATGTAGTCCAGCAAAATGACAATCTTATTCTCAC
TAATGTCAAGCTTGGTGAGGTTGGACAGTCCTGTGAATACTCCTAAAGGGACCAACTTAAGGCGATTGCC
TTTTAGGCGGAGGGAACGCAGGTTAAAGAGATTGTTAAATGCCCCAGGCTCCACATTGGCAATAATGTTG
TCGCTCAAGTCTATCTCCTCCAACAGAGGATATGAGATGAACTCTTCAGGGTTTATGCTCTTTAGTCGAT
TTTTGCTCAGGTCCAAGATTTTGGTCTCAATGGGAATGCCTTCTGGGATCGCGAGCAATCGTCTTCTGTG
GCAGCT
|
| nt 158094 - nt 158729 of AL844208.5 |
Notes: | The Lingo2 (LERN3) probe used in this study by Haines & Rigby, 2008 [PMID:18297755] "was obtained by PCR with Pfu polymerase (Stratagene) using primers 5'-AGCTGCCACAGAAGACGATT and 5'-GGACGTGAGGTTGAGACCAT from the BAC RP23-354D14 which contains the Lingo2/LERN3 gene. The PCR product was cloned into EcoRV digested pBluescript II KS yielding the plasmid LERN3 RT1. Antisense riboprobes were generated by digesting LERN3 RT1 with SalI and transcribing with T7 RNA polymerase". |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |