Type: | in situ hybridisation probe |
Identifier: | MGI:3789600 |
Entity Detected: | Lingo3, leucine rich repeat and Ig domain containing 3 ( MGI:3609246) |
Sequence: | sense strand is shown
>MGI:3789600
GTGGCGGCGGCGGTGGCAGTGGCGGCCGTGGCGGCGGCTGGTGGCGGCTCCGAGCAGCCCCTCGCGCCGT
CCGAGGAGGAGCCGCCCAGCTGTCCTGGACTATGCTGGCGTGGCCCTAGGCCCCACACCTTGCACCATGA
CCTGCTGGCTGCACATGCTGGGCCTGCACTTGCTGCTGCTGCCCACAGCGCCCCTGGCCGCAGGCTGCCC
CGCACGCTGCGAGTGCTCCGCATCCACCCGCACTGTGGCCTGTGGGCGCCGCCGGCTGACAGCCATCCCC
GAGGGCATCCCGGCCGAAACTCGCATGCTGGAGCTGAGCCGCAACCGCATCCGCTGTCTGAACCCTGGGG
ACCTGGCCTCGTTCCCCACCCTGGAGGAGCTGGACCTCAACCATAATGTGATTGCCCACGTGGAACCTGG
GGCCTTCGCCAACCTGCCCCGCCTGCGCGTCCTGCGTCTCCGTGGCAACCAGCTGAAGCTCATCCCGCCA
GGCGTGTTCACGCATCTGGACAGCCTCACGCTGCTGGACCTGAGCGAGAACAAGCTGGTCATCCTGCTGG
ATTTCAGCTTCCAGGACCTGCGCAGCCTGCAGCGGCTGGAGGTGGGCGACAATGACCTGGTGTTCATCTC
CCGCAGGGCCTTTGCGGGGCTGTTGGGGCTGGCTGAGCTCACGCTGGAGCGCTGCAATCTCACATCACTG
TCCCCGGAGTCGCTGGGTCACCTGCGGGGCTTGGGCGCTCTGCGCCTGCGCCACCTGGCCATCGCCGCCC
TGGAGGACCAGAATTTCCAGA
|
| nt 1 - nt 791 of BC072620.1 |
Notes: | The Lingo3 probe used in this study by Haines & Rigby, 2008 [PMID:18297755] "was obtained from IMAGE clone 6815693. A clone for riboprobe synthesis was obtained by digesting with EcoRI and HindIII and ligating into pBluescript II KS. An antisense probe was generated by digesting with EcoRI and transcribing with T3 RNA polymerase."
Editor's note: IMAGE:6815693 was created by priming first strand cDNA synthesis with an oligo-dT primer containing a NotI site. Double stranded cDNA was subsequently ligated with an EcoRI adaptor, digested with NotI, and then cloned directionally into pYX-Asc vector. BC072620.1 is the full 3447bp insert sequence from IMAGE:6815693, which is cut by HindIII at positions 791 and 3029. pYX-Asc does not contain a HindIII site (see http://www.imagenes-bio.de/info/vectors/pYX-Asc.shtml). Therefore the EcoRI and HindIII cloned fragment corresponds to nt 1-791 of BC072620.1. |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |