Type: | in situ hybridisation probe |
Identifier: | MGI:3789344 |
Entity Detected: | Lingo4, leucine rich repeat and Ig domain containing 4 ( MGI:2444651) |
Sequence: | sense strand is shown
>MGI:3789344
ACTGCAGTGGCTTGTGACAGTCAGGAAGGTGGTGGAGGCTCCTGCAGGCAGAGGCCACACTCCCCTGCAT
GCACTGGAATCCTTGGCTGGAATCCCTTCCTGTACCTAAGGGCACTGGCTGGATTGGGTACCGGGTAGCA
GAAAAGAACGGGAGCCTGCCTGCCTGCCTGCCTGCCTGCCTGCCTGCCTTGGAGCAGACTCTAAAAAGCT
GGAGATGCTTCTCAGCTCCACTTCTGAAACAATCTTGTGGTTGTGTTTGGCACCTGGTCCTGGCTATTAG
AGGATATACAGACATGGAGAGCAGACCGGAGCCTTGTCAGCTTTCCTGCCTTGTGTGAGTGGGTGCCTGG
GATCAGGGGATGTGCTGGCGATGAGGTCTGTGTCTGCAGTTCCCTGGGTCCCTCAGAGAAGATGCAGCAA
TGCCATGCTCAGACTAGAGCAGCAATCATAATGCAGGCGACAGGTGTGGGAAGGCTGTGCTGTTTGGCAT
GAAGCTGTGGTTCAGAGACCTTAGTCCTACTTCTCCCTTTGGCTTCTGAAGTGGACTTGGGTGGTCTTTA
GCATGGATGGAAAAGTCAGAATAACTTGGCAGTGACCCG
|
| nt 132465 - nt 133063 of AC122808.3 |
Notes: | The Lingo4 probe used in this study by Haines & Rigby, 2008 [PMID:18297755] "was obtained by PCR with Pfu polymerase (Stratagene) using primers 5'-CGGGTCACTGCCAAGTTATT and 5'-ACTGCAGTGGCTTGTGACAG from the BAC RP23-298M23 which contains the Lingo4/LERN4 gene. The PCR product was cloned into EcoRV digested pBluescript II KS yielding the plasmid LERN4 RT1. Antisense riboprobes were generated by digesting LERN4 RT1 with HindIII and transcribing with T7 RNA polymerase. |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |