EMAGE mouse gene expression spatial database

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EMAGE:5942

Capn2 calpain 2 ( MGI:88264)
TS17 (10.5 dpc)
in situ hybridisation

Data Images


Figure 2C. [doi:10.1387/ijdb.072448fr] Raynaud F; Marcilhac A; Chebli K; Benyamin Y; Rossel M, "Calpain 2 expression pattern and sub-cellular localization during mouse embryogenesis." Int J Dev Biol 2008; 52: 383-8. [PMID:18415939]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

5942_wholemount__possible.wlz

5942_wholemount__strong.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:5942_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

branchial arch

strong

trunk somite

strong

limb

strong

embryo

detected

Capn2 mRNA labelling was found at high levels within the whole embryo with particularly strong labelling of the limb buds, the branchial arches and somites.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Capn2 probeA
Entity Detected:	Capn2, calpain 2 ( MGI:88264)
Sequence:	sense strand is shown >Capn2 probeA ATCATCCCCGCAGCAGCGGCTCCCGCAGTCCGCTGCAGCGCCCCGGGCCCGGCCGCGCCCCAGCCGAGTG CCGTGCCCCGCCTCTCCGCGACCCCTTTCTCCGTGCAGTGCGCCAACCGGATCGCTACCATGGCGGGCAT CGCGATAAAGCTGGCCAAGGACCGCGAAGCGGCCGAGGGGCTGGGGTCGCATGAGAGAGCCATCAAGTAC CTCAACCAGGACTACGAGACGCTGCGGAACGAGTGCCTGGAGGCCGGGGCGCTCTTCCAGGATCCTTCCT TCCCCGCCCTGCCGTCATCCTTGGGCTATAAGGAGTTGGGGCCCTACTCCAGCAAAACTCGGGGCATCGA ATGGAAGCGGCCCACGGAGATCTGCGCTGACCCCCAGTTCATTATTGGAGGAGCCACCCGCACAGACATC TGCCAAGGAGCCCTGGGGGACTGCTGGCTGCTGGCTGCCATCGCCTCCCTCACCTTGAATGAGGAAATCC TGGCTCGGGTTGTGCCTCCAGATCAGAGCTTCCAGGAAAACTATGCAGGCATCTTCCACTTCCAGTTCTG GCAATATGGCGAGTGGGTGGAGGTGGTGGTGGACGACAGGCTGCCCACTAAGGACGGGGAGCTGCTCTTT GTGCATTCAGCTGAAGGGAGTGAGTTCTGGAGTGCCCTTCTGGAGAAAGCCTATGCCAAGATCAATGGGT GCTATGAAGCACTCTCAGGGGGTGCCACCACGGAAGGCTTTGAAGACTTCACAGGAGGCATTGCTGAGTG GTATGAGCTGAGGAAGCCTCCTCCCAACTTGTTCAAGATCATCCAGAAGGCTTTGGAGAAAGGTTCTCTG CTTGGCTGCTCTATCGATATCACCAGCGCTGCAGATTCTGAGGCCGTTACGTACCAGAAGTTGGTGAAAG GACATGCGTACTCTGTCACCGGAGCCGAGGAGGTTGAAAGTTCAGGAAGCCTGCAGAAATTGATCCGCAT CAGGAATCCCTGGGGACAAGTAGAGTGGACCGGGAAGTGGAATGACAATTGCCCCAGCTGGAACACGGTT GACCCAGAAGTAAGGGCAAATCTAACCGAACGGCAGGAGGACGGAGAATTCTGGATGTCCTTCAGTGACT TCCTGAGACACTACTCTCGCCTGGAGATCTGCAACCTGACCCCGGACACCCTCACCTGTGACTCCTATAA GAAGTGGAAACTCACCAAGATGGATGGGAACTGGAGGCGAGGCTCCACCGCAGGGGGCTGCAGGAATTAC CCAAATACCTTCTGGATGAACCCTCAGTACCTAATTAAGCTGGAGGAAGAAGACGAAGATGAGGAGGATG GGGAGAGAGGCTGTACCTTCCTGGTGGGTCTCATCCAGAAGCACCGGCGACGGCAGAGGAAGATGGGCGA GGACATGCACACCATTGGCTTCGGCATCTATGAGGTTCCAGAGGAGCTAACAGGGCAGACCAACATCCAC CTCGGCAAAAACTTTTTCCTCACAACCCGAGCCAGGGAGCGGTCAGATACCTTCATTAACCTCCGCGAGG TCCTCAACCGCTTCAAGCTGCCCCCGGGAGAGTATGTCCTCGTTCCCTCCACCTTCGAACCCCACAAGGA TGGCGATTTCTGCATCCGAGTCTTCTCGGAGAAGAAGGCTGACTACCAAGCTGTTGATGACGAGATCGAG GCCAACATTGAAGAGATTGACGCCAATGAGGAGGACATTGACGATGGATTCCGAAGGCTCTTTGTTCAGC TGGCTGGAGAGGATGCAGAGATCTCTGCCTTTGAGCTGCAGACCATCTTAAGAAGAGTTCTAGCCAAACG CCAAGACATCAAGTCAGACGGCTTCAGCATCGAGACCTGTAAGATCATGGTGGACATGCTGGATGAAGAT GGGAGTGGCAAGCTTGGCCTGAAGGAGTTCTACATCCTCTGGACGAAGATTCAGAAATACCAAAAAATCT ACCGGGAAATCGATGTGGACAGGTCTGGAACCATGAATTCCTACGAGATGCGGAAAGCACTGGAAGAAGC AGGTTTCAAGCTGCCCTGTCAACTTCATCAAGTCATCGTTGCCCGGTTTGCAGACGACGAGCTAATCATC GACTTTGACAATTTTGTGCGGTGTTTGGTCCGTCTGGAAACGCTATTCAAGATATTCAAGCAGCTAGACC CTGAGAACACTGGAACGATACAGCTCAACCTTGCTTCGTGGCTGAGTTTTTCAGTACTCTGAAGTTACAT GCCTGGAGAGGTCTAGTGGAAGAAAACAGCCAATGACTACACTTAGACACTTTGTATCTGGACCTTGAAC TTACGGGAACATTGACTTCATCGGATGATTATAACTGAAGAATATAACCGTCTGGGATAGATGAACTTTT CACATATCAGAGTAACATATTTGCATATTATTAAACCAAATGCAAATGAATTGCATAACCCTTGGTAAGG TCAGAGGAAGCGTTACACCTGTAAATAGTATATCCTTTTTACTTCTACACATTGTCCTGGCCATAGCAAT ATTAAATCAGGAAGGAATGCAGAGAGCTTTAAATAACTGGGCAAATAGTAAACCAATCTCAGAGGCTACA AGGCCTTGATGGGAATATTTGAAGAAATTTTAACTTTTAAATGCAGTGGTGGCACCTGCCACAGCAGCCC AGAGACTGCCGCTTGCCCTGAGCGAACTGAGAAATACAATGACGAAGTAGTCTGATGGAGGCATCTAAAG AACCCAGTCTGATGGAGGCATCTAAAGAATCCAGGAGCCAGGAGTGTCTGACACAAGGCCAACCTGGGCT GCCTAGCAAGACCCGATCTCAGAAAATAAATGCCAATTTAAAAAAAGGGCATGCTTGCTTCCATCATTTG GTTCTAACAGAAGATGGTTTTTATATACCTTTAGCATTCTTAAACTCAAGTCCTTGACCATATTTGGCCT TAGACACTTAGGTCTGACAAGTAGTAAGTAATGAGATCACTCTGACCAACTGAGCCAGGTGTGCCGTCTG CAAGAAGATCGTGATAAACCCCAGGGACTGTGCCTACAGCTGGGGAGGACAGGGCTCAGAAGATCTTCTT AAAACAACCAAGCTGGCTTCTTCATCCTGGGAAAATGCTAGCAACCATACAGACATATTGCCTTATCTAC TTCCAAACGTACTGTTAATAAAGAGTTACAACACAAAAAAAAAAAAAAAAAAAAA
	nt 1 - nt 3205 of BC054726.1
Notes:	The Capn2 probe used in this study by Raynaud et al., 2008 [PMID:18415939] was generated from "the sequence verified clone IRAVp968F10124D6 containing the mouse cDNA", which was "obtained from the RZPD resource center." Editors note: IRAVp968F10124D6 = IMAGE:5703563, the entire (3205bp) insert sequence of which is BC054726.1
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	10.5 dpc
Theiler Stage:	TS17
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Staining procedure:

alkaline phosphatase + BMpurple

General Information

Authors:	Raynaud F; Marcilhac A; Chebli K; Benyamin Y; Rossel M, 2008 [PMID:18415939] . Indexed and spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1387/ijdb.072448fr] [ PMID:18415939] Raynaud F, Marcilhac A, Chebli K, Benyamin Y, Rossel M 2008 Calpain 2 expression pattern and sub-cellular localization during mouse embryogenesis. Int J Dev Biol (52):383-8
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source