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EMAGE:5944

Cdca8 cell division cycle associated 8 ( MGI:1196274)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5944
Figure 2B. [doi:10.1007/s11033-008-9220-9] Zhang Q; Lin G; Gu Y; Peng J; Nie Z; Huang Y; Lu G, "Borealin is differentially expressed in ES cells and is essential for the early development of embryonic cells." Mol Biol Rep 2008 Mar 3. [PMID:18311593]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Both embryos hybridised with anti-sense and sense probes are shown in the paper.
Expression Pattern Description
Spatial Annotation:
EMAGE:5944Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5944_wholemount__possible.wlz
5944_wholemount__notDetected.wlz
5944_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5944_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
gut
not detected not detected
homogeneous
embryo
detected detected
Expressed everywhere except gut.
future brain
strong strong
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Cdca8 probeA
Entity Detected:Cdca8, cell division cycle associated 8 ( MGI:1196274)
Sequence:sense strand is shown

>Cdca8 probeA
TGCCTTCCATCCAAGAAGAGAACCCAGTCCATACAAGGAAGAGGCAGAAGTAAAAGGTTAAGCCATGACT
TTGTGACGCCAGCTATGAGCAGGCTGGAGCCGTCTCTGGTGAAACCAACCCCAGGCATGACACCTAGGTT
TGACTCCCGGGTCTTCAAGACTCCAGGGCTACGCACTCCAGCAGCCAAAGAGCAAGTTTACAACATCTCC
ATCAACGGCAGCCCTCTCGCAGACAGCAAAGAGATCTCCCTCAGTGTGCCCATAGGTGGCGGTGCGAGCT
TGCGGTTATTGGCCAGTGACTTGCAAAGGATTGATATTGCTCAGCTGAATCCAGAGGCCCTGGGAAACAT
TAGAAAGCTCTCGAGCCGCCTCGCCCAGATCTGCAGCAGCATACGGACGGGCCGATGAGAGGACAACAGG
ACACACAGTGGCAGCAGGGACTGTGGTAGCAGAGTGCACACATCTGTCCTTCTTCTGTGGGGTCCTTCAC
TGCCAACACCT
nt 684 - nt 1184 of NM_026560.3
Notes:The Cdca8 (Borealin) probe used in this study by Zhang et al., 2008 [PMID:18311593] was generated by RT-PCR using total RNA from mouse p19 cells and the following primers: forward: 5'-TGCCTTCCATCCAAGAAGAG-3'; reverse, 5'-AGGTGTTGGCAGTGAAGGAC-3'. The DNA amplified by PCR was cloned into pGEM-T Easy vector (Promega) and sequenced. The digested target plasmid was used to generate digoxygenin-labeled sense and antisense riboprobes by transcription using T7 and Sp6 RNA polymerase, respectively, according to the manufacturer's protocol.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Zhang Q; Lin G; Gu Y; Peng J; Nie Z; Huang Y; Lu G, 2008 [PMID:18311593] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1007/s11033-008-9220-9] [ PMID:18311593] Zhang Q, Lin G, Gu Y, Peng J, Nie Z, Huang Y, Lu G 2009 Borealin is differentially expressed in ES cells and is essential for the early development of embryonic cells. Mol Biol Rep (36):603-9
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE