Type: | in situ hybridisation probe |
Identifier: | Cdca8 probeA |
Entity Detected: | Cdca8, cell division cycle associated 8 ( MGI:1196274) |
Sequence: | sense strand is shown
>Cdca8 probeA
TGCCTTCCATCCAAGAAGAGAACCCAGTCCATACAAGGAAGAGGCAGAAGTAAAAGGTTAAGCCATGACT
TTGTGACGCCAGCTATGAGCAGGCTGGAGCCGTCTCTGGTGAAACCAACCCCAGGCATGACACCTAGGTT
TGACTCCCGGGTCTTCAAGACTCCAGGGCTACGCACTCCAGCAGCCAAAGAGCAAGTTTACAACATCTCC
ATCAACGGCAGCCCTCTCGCAGACAGCAAAGAGATCTCCCTCAGTGTGCCCATAGGTGGCGGTGCGAGCT
TGCGGTTATTGGCCAGTGACTTGCAAAGGATTGATATTGCTCAGCTGAATCCAGAGGCCCTGGGAAACAT
TAGAAAGCTCTCGAGCCGCCTCGCCCAGATCTGCAGCAGCATACGGACGGGCCGATGAGAGGACAACAGG
ACACACAGTGGCAGCAGGGACTGTGGTAGCAGAGTGCACACATCTGTCCTTCTTCTGTGGGGTCCTTCAC
TGCCAACACCT
|
| nt 684 - nt 1184 of NM_026560.3 |
Notes: | The Cdca8 (Borealin) probe used in this study by Zhang et al., 2008 [PMID:18311593] was generated by RT-PCR using total RNA from mouse p19 cells and the following primers: forward: 5'-TGCCTTCCATCCAAGAAGAG-3'; reverse, 5'-AGGTGTTGGCAGTGAAGGAC-3'. The DNA amplified by PCR was cloned into pGEM-T Easy vector (Promega) and sequenced. The digested target plasmid was used to generate digoxygenin-labeled sense and antisense riboprobes by transcription using T7 and Sp6 RNA polymerase, respectively, according to the manufacturer's protocol. |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |