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EMAGE:5958

Esam endothelial cell-specific adhesion molecule ( MGI:1916774)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5958
Figure 3A. [doi:10.1074/jbc.M100630200] Hirata Ki; Ishida T; Penta K; Rezaee M; Yang E; Wohlgemuth J; Quertermous T, "Cloning of an immunoglobulin family adhesion molecule selectively expressed by endothelial cells." J Biol Chem 2001; 276: 16223-31. [PMID:11279107]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5958Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5958_wholemount__moderate.wlz
5958_wholemount__weak.wlz
5958_wholemount__notDetected.wlz
5958_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5958_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
branchial arch artery
strong strong
regionalExpression is in the vascular endothelial cells including dorsal aorta, branchial arches, and intersomitic vessels.
dorsal aorta
strong strong
regionalExpression is in the vascular endothelial cells including dorsal aorta, branchial arches, and intersomitic vessels.
outflow tract
strong strong
regionalExpression is in the vascular endothelial cells including dorsal aorta, branchial arches, and intersomitic vessels.
cardiovascular system
strong strong
regionalExpression is in the vascular endothelial cells including dorsal aorta, branchial arches, intersomitic vessels and blood vessels.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3693932
Entity Detected:Esam, endothelial cell-specific adhesion molecule ( MGI:1916774)
Sequence:sense strand is shown

>MGI:3693932
CAAAGTGCTGGTTCCTCCAGCTCCTCCATCCTGTAGTTTACAGGGTGTACCCTATGTCGGGACCAATGTG
ACCCTGAACTGCAAGTCCCCAAGGAGTAAACCTACTGCTCAGTACCAGTGGGAGAGGCTGGCCCCATCCT
CCCAGGTCTTCTTTGGACCAGCCTTAGATGCTGTTCGTGGATCTTTAAAGCTCACTAACCTTTCCATTGC
CATGTCTGGAGTCTATGTCTGCAAGGCTCAAAACAGAGTGGGCTTTGCCAAGTGCAACGTGACCTTGGAC
GTGATGACAGGGTCCAAGGCTGCAGTGGTCGCTGGAGCAGTTGTGGGCACTTTTGTTGGGTTGGTGCTGA
TAGCTGGGCTGGTCCTGTTGTACCAGCGCCGGAGCAAGACCTTGGAAGAGCTGGCCAATGATATCAAGGA
AGATGCCATTGCTCCCCGGACCTTGCCTTGGACCAAAGGCTCAGACACAATCTCCAAGAATGGGACACTT
TCTTCGGTCACCTCAGCACGAGCT
nt 567 - nt 1080 of NM_027102.2
Notes:The Esam probe template used in this study by Hirata et al., 2001 [PMID:11279107] to generate S35 labelled probes is described as "a 514-base pair SacI mouse ESAM cDNA fragment", "cloned into pBluescript KS(+) ". Editors note: The template used to generate digoxigenin probes for whole-mount experiments is not explicitly described. SacI digestion of the mouse Esam cDNA RefSeq NM_027102.2 finds one fragment that matches the given information (spanning nt 566-1080 ).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Hirata Ki; Ishida T; Penta K; Rezaee M; Yang E; Wohlgemuth J; Quertermous T, 2001 [PMID:11279107] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1074/jbc.M100630200] [ PMID:11279107] Hirata Ki T, Ishida T, Penta K, Rezaee M, Yang E, Wohlgemuth J, Quertermous T 2001 Cloning of an immunoglobulin family adhesion molecule selectively expressed by endothelial cells. J Biol Chem (276):16223-31
Links:MGI:3693940 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI