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EMAGE:5966

Gdnf glial cell line derived neurotrophic factor ( MGI:107430)
TS17 (11 dpc)
in situ hybridisation

Data Images
EMAGE:5966
Figure 3F-E11. Copyright: Reprinted with permission from the Society for Neuroscience from [doi:10.1523/JNEUROSCI.4489-07.2008] J Neurosci 23; 28: 963-75. Baudet C; Pozas E; Adameyko I; Andersson E; Ericson J; Ernfors P, "Retrograde signaling onto Ret during motor nerve terminal maturation." Copyright 2008. [PMID:18216204]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotation: Arrowhead, otic vesicle.
Expression Pattern Description
Spatial Annotation:
EMAGE:5966Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5966_wholemount__moderate.wlz
5966_wholemount__possible.wlz
5966_wholemount__notDetected.wlz
5966_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5966_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
otocyst
detected detected
There is specific expression of GDNF mRNA in the otic vesicle
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Gdnf probeA
Entity Detected:Gdnf, glial cell line derived neurotrophic factor ( MGI:107430)
Notes:The Gdnf probe template used in this study by Baudet et al., 18216204 [PMID:18216204] was "amplified by reverse transcription (RT)-PCR" of the "full-length mouse GDNF" and "and either subcloned using a TOPO TA cloning kit from Invitrogen (Carlsbad, CA) or ligated into the PCDNA3 vector".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:11 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Baudet C; Pozas E; Adameyko I; Andersson E; Ericson J; Ernfors P, 18216204 [PMID:18216204] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1523/JNEUROSCI.4489-07.2008] [ PMID:18216204] Baudet C, Pozas E, Adameyko I, Andersson E, Ericson J, Ernfors P 2008 Retrograde signaling onto Ret during motor nerve terminal maturation. J Neurosci (28):963-75
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE