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EMAGE:5968

Gpi1 glucose phosphate isomerase 1 ( MGI:95797)
TS17 (9 dpc)
in situ hybridisation

Data Images
EMAGE:5968
Figure 1G. [doi:10.1111/j.1439-0264.2008.00865.x] Repiso A; Andres R; Climent F; Urena JM, "Expression patterns in mouse embryos of neuroleukin/glucose-6-phosphate isomerase and autocrine motility factor receptor." Anat Histol Embryol 2008; 37: 380-2. [PMID:18537943]

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5968Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5968_wholemount__moderate.wlz
5968_wholemount__possible.wlz
5968_wholemount__notDetected.wlz
5968_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5968_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
limb
detected detected
embryo
detected detected
regionalThe author states this gene is "expressed in vibrissae pads".
4th ventricle
detected detected
future spinal cord
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Gpi1 probeA
Entity Detected:Gpi1, glucose phosphate isomerase 1 ( MGI:95797)
Sequence:sense strand is shown

>Gpi1 probeA
GGATCCGGAGCGCTTCAACAACTTCAGCTTGAACCTCAACACCAACCATGGGCATATTCTGGTGGACTAC
TCCAAGAACCTTGTGAACAAGGAGGTGATGCAGATGCTGGTGGAGCTGGCCAAGTCCAGAGGCGTGGAGG
CTGCACGGGACAACATGTTCAGTGGTTCCAAGATCAACTACACCGAGGATCGGGCGGTGCTGCATGTGGC
CCTTCGGAACCGGTCCAACACACCCATCAAGGTGGACGGCAAAGATGTGATGCCGGAGGTGAACAGGGTT
CTGGACAAGATGAAGTCTTTCTGCCAGCGGGTCCGGAGTGGTGACTGGAAAGGGTACACTGGCAAATCCA
TCACGGACATCATCAACATCGGCATCGGGGGCTCTGACCTGGGACCCCTCATGGTGACTGAAGCTCTCAA
GCCTTACTCGAAAGGAGGTCCCCGTGTCTGGTTTGTCTCTAACATTGATGGGACCCACATTGCCAAAACA
CTGGCCAGCTTGTCCCCTGAGACTTCCCTCTTTATAATCGCCTCCAAGACCTTCACCACCCAGGAGACCA
TCACCAATGCAGAGACAGCAAAGGAGTGGTTTCTCGAAGCGGCCAAG
nt 145 - nt 751 of NM_008155.2
Notes:The Gpi1 (NK) probe used in this study by Repiso et al., 2008 [PMID:18537943] was "a 606-bp fragment corresponding to nucleotides 148-754 of the NK/GPI sequence was obtained by digestion with BamH1 and subcloned into pBluescript SK+. Sense riboprobe was generated by linearizing the plasmid with Not1 and performing transcription with T3 RNA polymerase, whereas antisense ripoprobe was obtained by Sal1 digestion and transcription with T7 RNA polymerase."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:OF1
Age:9 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Repiso A; Andres R; Climent F; Urena JM, 2008 [PMID:18537943] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1111/j.1439-0264.2008.00865.x] [ PMID:18537943] Repiso A, Andr�s R, Climent F, Ure�a JM 2008 Expression patterns in mouse embryos of neuroleukin/glucose-6-phosphate isomerase and autocrine motility factor receptor. Anat Histol Embryol (37):380-2
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE