Type: | in situ hybridisation probe |
Identifier: | Gpi1 probeA |
Entity Detected: | Gpi1, glucose phosphate isomerase 1 ( MGI:95797) |
Sequence: | sense strand is shown
>Gpi1 probeA
GGATCCGGAGCGCTTCAACAACTTCAGCTTGAACCTCAACACCAACCATGGGCATATTCTGGTGGACTAC
TCCAAGAACCTTGTGAACAAGGAGGTGATGCAGATGCTGGTGGAGCTGGCCAAGTCCAGAGGCGTGGAGG
CTGCACGGGACAACATGTTCAGTGGTTCCAAGATCAACTACACCGAGGATCGGGCGGTGCTGCATGTGGC
CCTTCGGAACCGGTCCAACACACCCATCAAGGTGGACGGCAAAGATGTGATGCCGGAGGTGAACAGGGTT
CTGGACAAGATGAAGTCTTTCTGCCAGCGGGTCCGGAGTGGTGACTGGAAAGGGTACACTGGCAAATCCA
TCACGGACATCATCAACATCGGCATCGGGGGCTCTGACCTGGGACCCCTCATGGTGACTGAAGCTCTCAA
GCCTTACTCGAAAGGAGGTCCCCGTGTCTGGTTTGTCTCTAACATTGATGGGACCCACATTGCCAAAACA
CTGGCCAGCTTGTCCCCTGAGACTTCCCTCTTTATAATCGCCTCCAAGACCTTCACCACCCAGGAGACCA
TCACCAATGCAGAGACAGCAAAGGAGTGGTTTCTCGAAGCGGCCAAG
|
| nt 145 - nt 751 of NM_008155.2 |
Notes: | The Gpi1 (NK) probe used in this study by Repiso et al., 2008 [PMID:18537943] was "a 606-bp fragment corresponding to nucleotides 148-754 of the NK/GPI sequence was obtained by digestion with BamH1 and subcloned into pBluescript SK+. Sense riboprobe was generated by linearizing the plasmid with Not1 and performing transcription with T3 RNA polymerase, whereas antisense ripoprobe was obtained by Sal1 digestion and transcription with T7 RNA polymerase." |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |