Type: | in situ hybridisation probe |
Identifier: | Gprin1 probeA |
Entity Detected: | Gprin1, G protein-regulated inducer of neurite outgrowth 1 ( MGI:1349455) |
Sequence: | sense strand is shown
>Gprin1 probeA
CCACGCGTCGACTAGTACGGGGGGGGGGAGGGGGGGGGGCCTCCCACAGCCTCAGAGATCAGAAATCCTG
TGTTCTCGGGGAAGATGGATGGCAACTCCCTGAAGCAGGCAGACTCCACTTCCACACGAAAGGAAGAGGC
TGGGTCCTTGAGGAATGAAGAGTCCATGTTGAAGGGAAAGGCAGAGCCTATGATCTATGGAAAGGGGGAG
CCTGGGACGGTAGGAAGAGTGGACTGCACAGCTTCTGGGGCGGAGAATTCTGGGTCCTTGGGAAAAGTAG
ACATGCCATGTTCCAGCAAAGTGGATATAGTGTCCCCAGGAGGAGACAATGCTGGGTCTTTAAGAAAGGT
AGAGACTATATCCTCAGGCAAAATGGATCCAAAGACAGAGAATGTCATGCATTCCAGAAGAGGGCGCCCT
GGATCCACAGGAGAGGGAGATCTTGTGTCTTTGAGGGAAAATGATATGAAACCCCCGGACAACACAGATT
CTGCCTCCACAAAAAAGACAGACCCTGAGTTCTCTGGAAAGCTAACTCCAGGATCGTCAGGCAAGACAGA
GCTTGTATCCTCAGTAACTGTGGCTCCTGTGACCTCTGAAAATGTGAATCCTGTATGCTCGGGGGGAGCA
GGTCCTGCAGCTGTGGGCAATTCAGAAACTTTGTCCTCAGTCAAGAAGGACCCTCAGTTGCTTGGAAAGA
AAGAGGCTGTCTCCTCAGGAGAAGGTGGGTCTGTATCGGTGAGAATGGCAGAAACAGTGTCTGCCAGACA
GCCAGAAGGTATGTTTCCAGCAAAGACAGATTCTACATCTTCCAACAGTACAGGACCTTCAGGCAGAGCG
GACCCTGTTTCCTTAAGAAATTCAGAACTCGTGTCCCCAGTGAAACCAGAACGCTTGTCCTCTGGGCAGG
CAGAACGCGTGTCCTTGGTAAAAACAGAAACATTATCCTCAGGAAAAGAAGATCCAAGGTCTTCTAGAAG
GGTGGATCACACGACTGTCACAGGAAACATGCAAACATCTCAAAAGGGGAATCCCGAGTCTTCAGGAAAG
ACAGACCTCGGGTCTTCGAGTTCAGGAGATACCAGGTCTCTGGGGACTTGGGGGTCCCTGTCTGCTGCAA
AAGCTGAGGTGACTGAGGGGAAAGGAGACCCACAGCCCTGGAAGAAGGCAAGTCTCCCAGCCTCAGAGAA
GACCGATCCCCTGGCTTCAAGCAAGGCAGGCTCTGCAAGCCAGGGAAAGGCAGAAACGGTTTCCCCTGGA
GAAGTGGACGCCATGACTTTAGGAAAAACGGTCCCAACGAGTTCAGGAAAAACAGCTTTGGTATCCCCAG
GGAAGGTGGATCTCATGACCTCAGAAAGAGCAGAAGGCATCCCAGAGCTGCAGGCTTCAGAGAAAGGGAA
TCCTGTTAACTCCACACGGGTGGACACCGGGGCCACAGGGAGTACTGAGCCCAAATCTGGGGTCAAAGTG
ATAACCCAGATTCCAGGTGCCACATCCCCGGGAAAGGTGGAAACTCCGTCTTTGCAAAAGGAGCAGCCAC
AACTGTCTGAGAAGACAGATCCCTCCAGAAAAGTCGACCCCCCCACGACTGTGGAGCCTGTGTCCCTGGG
GAAGGCTGACTCTGCATCTCCATCTCCTAGAAAAGCAGAGTCTCAGACTTCCGCCAAGACTGTCCCTCAG
GCCCCAGATAAGGCCACGTCCTCTCTCAGGCAATCAGATGGCACACCCTACAGCTCAGCCCAGCCCCAGA
GAGATACCAGGAGCATCGGATCCCTGCCAGAGCGGGAGCCCTCTGCCAGCACCAGCCAGAAAGACCTAGC
GGCAGCGGCGGCTCAGAAGAGCCCCAGCGCGGAGGCTGCGGCGCCCCCTCCAGGGCCACGGACTCGCGAC
AACTTCACCAAGGCGCCGTCGTGGGACGCCGGAGCGCCACCGCCACGCGAGGACGCGGGCACCCAGGCAG
GCGCGCAGGCCTGCGTCTCGGTGGCGGTGAGCCCCATGTCTCCGCAGGACGGCGCGGGTGGCCCGGCCTT
CAGCTTCCAGGCGGCGCCGCGCGCGCCCAGCCCCGCGCCCAGGCCACCATCGCGCCGGGACGCGGGCCTG
CAGGTGTCGCTGGGAGCCGCCGAGACACGCTCGGTGGCCACGGGGCCCATGACTCCGCAGGCCGCAGCGC
CGCCCGCCGTGCCGCCCGTCTTCCCCGAGGTGCGGGTGCGGCCAGGCTCGGTGTTGGCGGCCGCCTTGGC
GCCCCAGGAGGCGACCGAGCCGGTGCGCGACGTGAGCTGGGACGAGAAGGGCATGACGTGGGAAGTGTAC
GGTGCTTCCATGGAGGTGGAAGTGCTGGGCATGGCCATCCAGAAGCATTTGGAGCGTCAGATCGAGGAGC
ACGGCCGCCAAGGGGCGCCGGCGCCGGCGCCGCCGCCCGCCGTCCGCGCGGGCCCAGGCCGTGCAGGCTC
TGTGCGCACCGCACCGGCGGAGGGCGCCGCCAAGCGCCCGCCGGGCCTCTTCCGCGCGCTGCTGCAGAGT
GTGCGCCGGCCGCGGTGCTGCTCGCGGGCGGGCCCCACTGCGGAGTGATCTGTCCCTGTTTTGTAGGCCC
GGGTTTCCAACCTTCTCAGGCTCCCTTCTCGAGGTCTGGCCCTTGAAAGCTATGTTCCACCCCCAAGTGC
GTCATGCCTCCGAGGAGTGAGCCGCCTTCAGGGGCTTCTTTCAAGCCCTTCCTTCCCAAACACTGACGGA
AAACCCCATATCCAGTCCCTTTGTGGCCAGGAGCCCACACACCCAGCCCTGACAACTCCACCCTGGTTCC
CTCACAGACTTTTGCTCCACTCCCGCGGGAACTACGGGAAGAGCACAGGGACCTGCTCCCACCCTTTCAC
CCTACAGCTGAGGCAGGCTCCTGAGATGGCCAGACTGGTGGTTTGAGGCTTCCAGTGGGCCAGGTCCCCA
AAACGGGGAAAGACTGCATGCAGATCTTTAAGTGTTCTTTCACGTGTGCCAGGATTGATGTAAGGCCACC
AGAAAGGCAGACTCTAATAGGAGGAGTCTAGGGGGAGGGGAGGGAGTGAGTCTCTGAGGCAGTGAGGGTC
TCCACATGGTGCTGGCCCCGTGGCCAGGGCATCTGTTGCATGCTGTTGGTAGTCCTACCCCATCCCTGTC
TCTCCCCACCTAGCCCAGTACCCTAGTCCTATTTCCTCCCCATCCCAGTCACTATGCACAGTTCCCATAG
AGGCCAAAGGCTAGGAATGCCCTGGGGCTCAACTGAGGAGAGTCATGGAAAGGTCAGAGGGTCTTAGACA
GGACCAAAGGTCATTGTAAGGCTCCCAGAAGGTCTCAGGACGTCGACCACCTGGGTGACAGCTGGTCTTG
GGTATTGGGAACAGGATGGAGGTCCCATTTGATATCCACGGTTGTCTTTAAAGATTCCCCTCTTATCCCT
CTCCCCGACACGCACAATGCTGTGAGCCCCAAGTTCCTTGTAACCACTGAGTGCCCTGGTCCCTCTAGCC
TCCGTTTTTGGCTACTAACTGTCACAGTATACATTGGTAATAAAAACCTTTCCCCAAAAAAAAAAAAA
|
| nt 1 - nt 3568 of AF146569.1 |
Notes: | The Gprin1 (Grin1/Z-16) probe used in this study by Masuho et al., 2008 [PMID:18729205] was "prepared from pBS-GRIN1. For whole-mount ISH of GRIN1, pBS-GRIN1 linearized by Asp718 was transcribed with T7 RNA polymerase for antisense probes". pBS-GRIN1 was made by releasing a 3.6-kb mouse GRIN1 cDNA fragment from the pCMV5-GRIN1 expression vector (Chen et al.,1999 [PMID:10480904] ) by using KpnI and HindIII restriction enzymes and then cloning this fragment into KpnI-HindIII sites of pBluescriptII KS(-).
Editors note: the 3.6kb fragment reported by Chen et al is AF146569.1. Asp718, KpnI and HindIII do not cut this sequence indicating the probe was transcribed from its entire length. |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |