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EMAGE:5972

Grhl3 grainyhead-like 3 (Drosophila) ( MGI:2655333)
TS14 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5972
Fig 1A. [doi:10.1093/hmg/ddm221] Gustavsson P, Greene ND, Lad D, Pauws E, de Castro SC, Stanier P, Copp AJ. "Increased expression of Grainyhead-like-3 rescues spina bifida in a folate-resistant mouse model." Hum Mol Genet 2007 16(21):2640-6

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5972Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5972_wholemount__moderate.wlz
5972_wholemount__weak.wlz
5972_wholemount__notDetected.wlz
5972_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5972_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
prosencephalon
detected detected
regionalExpressed in ventral forebrain.
notochord
detected detected
regionalExpressed in caudal most region.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Grhl3 probeA
Entity Detected:Grhl3, grainyhead-like 3 (Drosophila) ( MGI:2655333)
Sequence:sense strand is shown

>Grhl3 probeA
GAGACGAGTGGTCAGCAGCGCCTGCGAGCCAACCAGAGACGGATCGCTGGAACCTCGGAGAAGGAAGATG
TCGAATGAACTTGATTTCAGGTCTGTGCGGTTGCTGAAGAATGACCCTGTGAGCTTCCAGAAGTTTCCCT
ACAGTAATGAGGACGAGGCCTGGAAGACATACCTGGAGAACCCTTTGACGGCTGCCACCAAAGCCATGAT
GAGAGTCAACGGGGACGAGGAGAGTGTGGCTGCTCTGAGCTTCCTCTACGACTACTATATGGGTCCCAAG
GAGAAGCGGATACTGTCCTCCAGCACTGGTGGCCGGAATGACCAAGGAAAGAAGTTCTACCACAGCATGG
ACTATGAGCCGGATCTTGCCCCCCTCGAGAGCCCCACACACCTCATGAAATTTTTGACAGAGAACGTGTC
TGGAAGTCCAGACTACACAGACCAGCTCAAGAAAAACAATCTGCTAGGCTTGGAGGGGGTTCTACCCACC
CCCGG
nt 130 - nt 624 of NM_001013756.1
Notes:The Grhl3 probe used in this study by Gustavsson et al., 2007 [PMID:17720888] "was complementary to a 494 bp fragment of Grhl3 corresponding to nucleotides 130-624 (GenBank accession number NM001013756)."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Gustavsson P; Greene ND; Lad D; Pauws E; de Castro SC; Stanier P; Copp AJ, 2007 [PMID:17720888] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1093/hmg/ddm221] [ PMID:17720888] Gustavsson P, Greene ND, Lad D, Pauws E, de Castro SC, Stanier P, Copp AJ 2007 Increased expression of Grainyhead-like-3 rescues spina bifida in a folate-resistant mouse model. Hum Mol Genet (16):2640-6
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE