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EMAGE:5973

Hba-x hemoglobin X, alpha-like embryonic chain in Hba complex ( MGI:96019)
TS14 (14 Somite no.)
in situ hybridisation

Data Images
EMAGE:5973
Figure 2B. [doi:10.1182/blood-2007-08-107086] Lux CT; Yoshimoto M; McGrath K; Conway SJ; Palis J; Yoder MC, "All primitive and definitive hematopoietic progenitor cells emerging before E10 in the mouse embryo are products of the yolk sac." Blood 2008; 111: 3435-8. [PMID:17932251]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5973Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5973_wholemount__moderate.wlz
5973_wholemount__weak.wlz
5973_wholemount__notDetected.wlz
5973_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5973_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
A significant amount of zeta-globin staining is detected in the embryo proper.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Hba-x probeA
Entity Detected:Hba-x, hemoglobin X, alpha-like embryonic chain in Hba complex ( MGI:96019)
Sequence:sense strand is shown

>Hba-x probeA
GAGAAGATGGCTGCTCAGGCCGAGCCCATTGGCACTGAGACTCTAGAGAGGCTCTTCTGCAGCTACCCCC
AGACGAAGACCTACTTCCCCCACTTCGACCTGCACCATGGGTCTCAGCAGTTGCGGGCCCACGGCTTCAA
GATCATGACCGCCGTAGGGGATGCGGTTAAGAGCATCGACAACCTCTCTAGTGCTTTGACTAAGCTGAGC
GAGCTGCATGCCTACATCCTGCGTGTGGATCCGGTCAACTTCAAGCTCCTGTCCCACTGTCTGCTGGTCA
CAATGGCCGCACGCTTTCCCGCCGACTTCACCCCTGAGGTCCACGAAGCCTGGGACAAGTTCATGTCTAT
CCTGTCTTCTATCCTGACTGAGAAGTACCGCTAAGCCTCCACCGCCACGACCCCCATGACCAGCTATGAT
CCCTCTCCTCCCCTTTATTGAACCCTCCCTCAGGTCTTTCC
nt 108 - nt 568 of NM_010405.3
Notes:The Hba-x (zeta-globin) probe used in this study by Lux et al., 2008 [PMID:17932251] corresponded "to nucleotides 108 to 568 of the embryonic zeta-globin mRNA (GenBank accession number NM_010405)". Editors note: It is presumed the authors are referring to version 3 of NM_010405 (i.e. NM_010405.3) as it was released on Apr 6 2007, i.e. 1 year prior to publication of this study on 1 April 2008.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:14 Somite no.
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Lux CT; Yoshimoto M; McGrath K; Conway SJ; Palis J; Yoder MC, 2008 [PMID:17932251] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1182/blood-2007-08-107086] [ PMID:17932251] Lux CT, Yoshimoto M, McGrath K, Conway SJ, Palis J, Yoder MC 2008 All primitive and definitive hematopoietic progenitor cells emerging before E10 in the mouse embryo are products of the yolk sac. Blood (111):3435-8
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE