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EMAGE:5974

Hif1a hypoxia inducible factor 1, alpha subunit ( MGI:106918)
TS17 (10.5 dpc)
immunohistochemistry

Data Images
EMAGE:5974 EMAGE:5974
Figure 1F. [doi:10.1083/jcb.200612023] Copyright: This image is from Provot S; Zinyk D; Gunes Y; Kathri R; Le Q; Kronenberg HM; Johnson RS; Longaker MT; Giaccia AJ; Schipani E, "Hif-1alpha regulates differentiation of limb bud mesenchyme and joint development." J Cell Biol 2007 May 7;177(3):451-64 [PMID:17470636] , and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License http://creativecommons.org/licenses/by-nc-sa/3.0/. Figure 1C. [doi:10.1083/jcb.200612023] Copyright: This image is from Provot S; Zinyk D; Gunes Y; Kathri R; Le Q; Kronenberg HM; Johnson RS; Longaker MT; Giaccia AJ; Schipani E, "Hif-1alpha regulates differentiation of limb bud mesenchyme and joint development." J Cell Biol 2007 May 7;177(3):451-64 [PMID:17470636] , and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License http://creativecommons.org/licenses/by-nc-sa/3.0/.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: expression in limb bud mesenchyme (asterisk) and apical ectoderm (arrowhead).
Expression Pattern Description
Spatial Annotation:
EMAGE:5974Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5974_wholemount__moderate.wlz
5974_wholemount__possible.wlz
5974_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5974_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
forelimb bud mesenchyme
detected detected
trunk somite
detected detected
Hif-1alpha protein is detected in the somites, giving rise to the axial skeleton
forelimb bud apical ectodermal ridge
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:Hif1a antibodyA
Entity Detected:Hif1a, hypoxia inducible factor 1, alpha subunit ( MGI:106918)
Notes:The anti-HIF1A antibody used in this study by Provot et al., 2007 [PMID:17470636] is described as "a primary antibody against Hif-1alpha (R&D Systems) diluted 1:100". Editor's note: R&D Systems sell several different anti-HIF-1 alpha antisera. It is not specified by the authors which of these were used.
Supplier:R&D Systems
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Provot S; Zinyk D; Gunes Y; Kathri R; Le Q; Kronenberg HM; Johnson RS; Longaker MT; Giaccia AJ; Schipani E, 2007 [PMID:17470636] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1083/jcb.200612023] [ PMID:17470636] Provot S, Zinyk D, Gunes Y, Kathri R, Le Q, Kronenberg HM, Johnson RS, Longaker MT, Giaccia AJ, Schipani E 2007 Hif-1alpha regulates differentiation of limb bud mesenchyme and joint development. J Cell Biol (177):451-64
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE