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EMAGE:5976

Hoxd9 homeobox D9 ( MGI:96210)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5976
Figure S3. [doi:10.1128/MCB.01168-07] Mol Cell Biol 2008 Jan;28(1):315-25 [PMID:17984226] Feng W; Huang J; Zhang J; Williams T, "Identification and analysis of a conserved Tcfap2a intronic enhancer element required for expression in facial and limb bud mesenchyme." � ASM. Reproduced with permission from Molecular and Cellular Biology. Reuse of any Licensed Material requires permission from ASM. Copyright 2008.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5976Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5976_wholemount__moderate.wlz
5976_wholemount__weak.wlz
5976_wholemount__notDetected.wlz
5976_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5976_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
forelimb bud
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3767654
Entity Detected:Hoxd9, homeobox D9 ( MGI:96210)
Notes:The Hoxd9 probe used in this study by Feng et al., 2008 [PMID:17984226] is described as being "generated using equivalent procedures from previously published plasmid reagents (Dolle et al., 1991 [1685889])". Editors note Dolle et al., refer to Hoxd9 as Hox-4.4. They mention a Hox-4.4 probe that was used as a positive control, but do not describe it.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Feng W; Huang J; Zhang J; Williams T, 2008 [PMID:17984226] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1128/MCB.01168-07] [ PMID:17984226] Feng W, Huang J, Zhang J, Williams T 2008 Identification and analysis of a conserved Tcfap2a intronic enhancer element required for expression in facial and limb bud mesenchyme. Mol Cell Biol (28):315-25
 [ doi:10.1016/0925-4773(91)90067-G] [ PMID:1685889] Dolle P, Izpisua-Belmonte JC, Boncinelli E, Duboule D 1991 The Hox-4.8 gene is localized at the 5' extremity of the Hox-4 complex and is expressed in the most posterior parts of the body during development. Mech Dev (36):3-13
Links:MGI:3789145 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI