Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5977

Hsd17b7 hydroxysteroid (17-beta) dehydrogenase 7 ( MGI:1330808)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5977
Figure 6 (left). [doi:10.1210/me.2008-0165] Shehu A; Mao J; Gibori GB; Halperin J; Le J; Devi YS; Merrill B; Kiyokawa H; Gibori G, "Prolactin receptor-associated protein/17beta-hydroxysteroid dehydrogenase type 7 gene (Hsd17b7) plays a crucial role in embryonic development and fetal survival." Mol Endocrinol 2008; 22: 2268-77. [PMID:18669642]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5977Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5977_wholemount__moderate.wlz
5977_wholemount__weak.wlz
5977_wholemount__possible.wlz
5977_wholemount__notDetected.wlz
5977_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5977_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
heart
detected detected
eye
detected detected
ear
detected detected
telencephalon
detected detected
future midbrain
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Hsd17b7 probeA
Entity Detected:Hsd17b7, hydroxysteroid (17-beta) dehydrogenase 7 ( MGI:1330808)
Sequence:sense strand is shown

>Hsd17b7 probeA
CCCTGCTGGCCTCTCACCCCTCCGCCGAAGTCAGCATCGTGCAGATGGATGTCAGCAGCCTGCAGTCGGT
GGTCCGGGGTGCAGAGGAAGTCAAGCAAAAGTTTCAAAGATTAGACTACTTATATCTGAATGCTGGAATC
CTGCCTAATCCACAATTCAACCTCAAGGCATTTTTCTGCGGCATCTTTTCAAGAAATGTGATTCATATGT
TCACCACAGCGGAAGGAATTTTGACCCAGAATGACTCGGTCACTGCCGACGGGTTGCAGGAGGTGTTTGA
AACCAATCTCTTTGGCCACTTTATTCTGATTCGGGAACTGGAACCACTTCTCTGCCATGCGGACAACCCC
TCTCAGCTCATCTGGACGTCCTCTCGCAATGCAAAGAAGGCTAACTTCAGCCTGGAGGACATCCAGCACT
CCAAAGGCCCGGAACCCTACAGCTCTTCCAAATATGCTACCGACCTCCTGAATGTGGCTTTGAACAGGAA
TTTCAACCAGAAGGGTCTGTATTCCAGTGTGATGTGCCCAGGCGTCGTGATGACCAATATGACGTATGGA
ATTTTGCCTCCCTTTATCTGGACGTTGCTCCTAC
nt 213 - nt 806 of NM_010476.3
Notes:The Hsd17b7 probe used in this study by Shehu et al., 2008 [PMID:18669642] was "part of the mouse HSD17B7 gene (bp 213-806; GenBank NM_010476)". Editors Note: The 3 versions of NM_010476 vary considerably in length (1756, 4528 and 4574 nt). It is assumed the authors are referring to NM_010476.3 as it was released on Feb 11 2006, approx 2 years prior to publication of this paper.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Shehu A; Mao J; Gibori GB; Halperin J; Le J; Devi YS; Merrill B; Kiyokawa H; Gibori G, 2008 [PMID:18669642] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1210/me.2008-0165] [ PMID:18669642] Shehu A, Mao J, Gibori GB, Halperin J, Le J, Devi YS, Merrill B, Kiyokawa H, Gibori G 2008 Prolactin receptor-associated protein/17beta-hydroxysteroid dehydrogenase type 7 gene (Hsd17b7) plays a crucial role in embryonic development and fetal survival. Mol Endocrinol (22):2268-77
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE