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EMAGE:5981

Igf1 insulin-like growth factor 1 ( MGI:96432)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:5981
Fig 5A. [doi:10.1242/dev.017624] Copyright: This image is from Kutejova E, Engist B, Self M, Oliver G, Kirilenko P, Bobola N Development 2008;135(8):1463-70 and is displayed with the permission of The Company of Biologist Limited who owns the Copyright. [PMID:18321982]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: I, first branchial arch; II, second branchial arch; the arrow indicates an area where Igf1 is upregulated in Hoxa2 homozygote mutants of similar age (see Fig 5B for the mutant).
Expression Pattern Description
Spatial Annotation:
EMAGE:5981Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5981_wholemount__moderate.wlz
5981_wholemount__notDetected.wlz
5981_wholemount__strong.wlz
5981_wholemount__weak.wlz
5981_wholemount__possible.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5981_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
regionalExpressed in a region "below the developing otic vesicle" - see arrow in image.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Igf1 probeB
Entity Detected:Igf1, insulin-like growth factor 1 ( MGI:96432)
Sequence:sense strand is shown

>Igf1 probeB
CATGTCGTCTTCACACCTCTTCTACCTGGCGCTCTGCTTGCTCACCTTCACCAGCTCCACCACAGCTGGA
CCAGAGACCCTTTGCGGGGCTGAGCTGGTGGATGCTCTTCAGTTCGTGTGTGGACCGAGGGGCTTTTACT
TCAACAAGCCCACAGGCTATGGCTCCAGCATTCGGAGGGCACCTCAGACAGGCATTGTGGATGAGTGTTG
CTTCCGGAGCTGTGATCTGAGGAGACTGGAGATGTACTGTGCCCCACTGAAGCCTACAAAAGCAGCCCGC
TCTATCCGTGCCCAGCGCCACACTGACATGCCCAAGACTCAGAAGGAAGTACATTTGAAGAACACAAGTA
GAGGAAGTGCAGGAAACAAGACCTACAGAATGTAGG
nt 152 - nt 537 of X04480.1
Notes:The Igf1 probe template used in this study by Kutejova et al., 2008 [PMID:18321982] is indicated as that used by Weger and Schlake, 2005 [PMID:16297183] . i.e. a fragment generated by PCR using the following gene-specific primers: Igf-I: nt. 152#8211;170 and nt. 518#8211;537 in X04480 (386 bp).
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Kutejova E; Engist B; Self M; Oliver G; Kirilenko P; Bobola N, 2008 [PMID:18321982] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.017624] [ PMID:18321982] Kutejova E, Engist B, Self M, Oliver G, Kirilenko P, Bobola N 2008 Six2 functions redundantly immediately downstream of Hoxa2. Development (135):1463-70
 [ doi:10.1111/j.0022-202X.2005.23946.x] [ PMID:16297183] Weger N, Schlake T 2005 Igf-I signalling controls the hair growth cycle and the differentiation of hair shafts. J Invest Dermatol (125):873-82
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE