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EMAGE:5983

Ihh Indian hedgehog ( MGI:96533)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5983
Fig4A. [doi:10.1002/dvdy.21536] Copyright: This image is from Fogel JL; Chiang C; Huang X; Agarwala S, "Ventral specification and perturbed boundary formation in the mouse midbrain in the absence of Hedgehog signaling." Dev Dyn 2008; 237: 1359-72. Reprinted with permission of Wiley-Liss Inc. [PMID:18429041]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotation: HG, hindgut. Editor's note: This embryo has been denoted Shh+/? and therefore may either be a heterozygote mutant or a wild type.
Expression Pattern Description
Spatial Annotation:
EMAGE:5983Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5983_wholemount__possible.wlz
5983_wholemount__notDetected.wlz
5983_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5983_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
hindgut
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Ihh probeB
Entity Detected:Ihh, Indian hedgehog ( MGI:96533)
Notes:The Ihh probe used in this study by Fogel et al., 2008 [PMID:18429041] is of unreported sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:Shhtm1Chg/Shh+
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Fogel JL; Chiang C; Huang X; Agarwala S, 2008 [PMID:18429041] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.21536] [ PMID:18429041] Fogel JL, Chiang C, Huang X, Agarwala S 2008 Ventral specification and perturbed boundary formation in the mouse midbrain in the absence of Hedgehog signaling. Dev Dyn (237):1359-72
Links:MGI:1857796 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE