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EMAGE:5984

Insm1 insulinoma-associated 1 ( MGI:1859980)
TS17 (10.5 dpc)
in situ reporter

Data Images
EMAGE:5984
Fig1B. [doi:10.1242/dev.011783] Copyright: This image is from Wildner H; Gierl MS; Strehle M; Pla P; Birchmeier C, "Insm1 (IA-1) is a crucial component of the transcriptional network that controls differentiation of the sympatho-adrenal lineage." Development 2008 135:473-81 and is displayed with the permission of The Company of Biologist Limited who owns the Copyright. [PMID:18094025]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotation: primary sympathetic ganglion chain (arrowhead).
Expression Pattern Description
Spatial Annotation:
EMAGE:5984Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5984_wholemount__moderate.wlz
5984_wholemount__weak.wlz
5984_wholemount__notDetected.wlz
5984_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5984_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
sympathetic nervous system
detected detected
Expressed along the entire primary sympathetic ganglion chain.
central nervous system
detected detected
central nervous system ganglion
detected detected
Detected in the sensory ganglia
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ reporter
Entity Detected:Insm1, insulinoma-associated 1 ( MGI:1859980)
Notes:Insm1 expression was assayed in this study by Wildner et al., 2008 [PMID:18094025] in embryos heterozygous for a targeted allele where an NLS-lacZ gene followed by a self-excising protamine-cre neomycin selection cassette was fused in-frame to the initiation codon of the gene. Following passage through the male germline of the founder chimeric mouse, the neomycin selection cassette was deleted, leaving the lacZ gene and a single loxP site in place. Beta-galactosidase is expressed from this allele under the control of the endogenous promoter.
Labelled with:LacZ
Specimen
Organism:mouse
Strain:involves: 129P2/OlaHsd * C57BL/6
Age:10.5 dpc
Theiler Stage:TS17
Mutations:Insm1tm1Cbm/Insm1+
Preparation:wholemount
Procedures
General Information
Authors:Wildner H; Gierl MS; Strehle M; Pla P; Birchmeier C, 2008 [PMID:18094025] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.011783] [ PMID:18094025] Wildner H, Gierl MS, Strehle M, Pla P, Birchmeier C 2008 Insm1 (IA-1) is a crucial component of the transcriptional network that controls differentiation of the sympatho-adrenal lineage. Development (135):473-81
Links:MGI:3656035 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE