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EMAGE:5986

Jazf1 JAZF zinc finger 1 ( MGI:2141450)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:5986
Figure 1Q. [doi:10.1111/j.1525-142X.2004.04050.x] Lehoczky JA; Williams ME; Innis JW, "Conserved expression domains for genes upstream and within the HoxA and HoxD clusters suggests a long-range enhancer existed before cluster duplication." Evol Dev 2004; 6: 423-30. [PMID:15509224]

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5986Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5986_wholemount__possible.wlz
5986_wholemount__notDetected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5986_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
weak weak
low-level, ubiquitous, embryonic expression including the limb and the genital bud
limb
weak weak
genital tubercle
weak weak
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Jazf1 probeA
Entity Detected:Jazf1, JAZF zinc finger 1 ( MGI:2141450)
Sequence:sense strand is shown

>Jazf1 probeA
GGAGTCAGACAGTGATGAGTCCTGGACCACAGAGAGCGCCATCAGCTCTGAAGCAATCCTCAGCTCCATG
TGCATGAATGGAGGGGAAGAGAAGCCTTTCGCCTGCCCAGTTCCAGGGTGTAAAAAGAGATACAAGAATG
TGAATGGCATAAAGTACCATGCTAAGAATGGTCACCGAACACAGATTCGCGTCCGCAAACCATTCAAATG
CCTGTGTGGGAAGAGTTACAAGACAGCTCAGGGCCTGCGGCACCACACAATCAATTTCCATCCCCCAGTG
TCTGCCGAGATGATCAGGAAGATGCAGCAGTAGCGTGCTGGTCAGAACTGTGCCAAGAAATCCTCACCAG
CAGTTGCTGATTTTGAAAACAGCCACCTTTTTTCAGGGGAAGCACTCAGCAACCCTTTAATGAAAAATAA
TTAAATGCATGCTTTAACCTTTTCTGTACTTTTGGAATGATGTATCTTTGTAGTTAATGATTTTGTACAT
TTGCACATGTAATCATCACGCCCATTTCCATTACGTTGATATAAGGTGCTAAACAAAACAAGCTCTAGGT
TCTTCAGCACATTTTCCCCAAAACAAAATAAATGAAGGGCATGTTGCATATTGTTTAACC
nt 448 - nt 1067 of NM_173406.2
Notes:The Jazf1 (Jaz1) probe used in this study by Lehoczky et al., 2004 [PMID:15509224] was "amplified from an E12.5 C57Bl/6 mouse limb bud cDNA library and cloned into polymerase chain reaction-4 Topo vector (Invitrogen, Carlsbad, CA, USA)". The primers used for the amplification were jaz1.F 5'-GGAGTCAGACAGTGATGAGTCC and jaz1.R 5'-GGTTAAACAATATGCAACATGCC.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Lehoczky JA; Williams ME; Innis JW, 2004 [PMID:15509224] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1111/j.1525-142X.2004.04050.x] [ PMID:15509224] Lehoczky JA, Williams ME, Innis JW 2004 Conserved expression domains for genes upstream and within the HoxA and HoxD clusters suggests a long-range enhancer existed before cluster duplication. Evol Dev (6):423-30
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE