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EMAGE:5997

Rasgrp3 RAS, guanyl releasing protein 3 ( MGI:3028579)
TS17 (10.5 dpc)
in situ reporter

Data Images
EMAGE:5997
Figure 4B of [doi:10.1128/MCB.24.24.10515-10528.2004] Mol Cell Biol 2004; 24: 10515-28 [PMID:15572660] Roberts DM; Anderson AL; Hidaka M; Swetenburg RL; Patterson C; Stanford WL; Bautch VL, "A vascular gene trap screen defines RasGRP3 as an angiogenesis-regulated gene required for the endothelial response to phorbol esters." � ASM. Reproduced with permission from Molecular and Cellular Biology. Reuse of any Licensed Material requires permission from ASM. Copyright 2004.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: arrow indicates expression in the pericardium.
Expression Pattern Description
Spatial Annotation:
EMAGE:5997Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5997_wholemount__moderate.wlz
5997_wholemount__weak.wlz
5997_wholemount__possible.wlz
5997_wholemount__strong.wlz
5997_wholemount__notDetected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5997_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
dorsal aorta
strong strong
cardiovascular system
detected detected
Expressed in the developing intersomitic vessels and majority of head vessels and small vessels along the sides of the embryo also faintly express Rasgrp3 as well as the pericardium.
trunk somite
detected detected
regionalExpressed in myotome.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ reporter
Entity Detected:Rasgrp3, RAS, guanyl releasing protein 3 ( MGI:3028579)
Notes:Rasgrp3 expression was assayed in this study by Roberts et al., 2004 [PMID:15572660] in embryos heterozygous for a gene trapped allele where the gene trap vector containing lacZ and its own start codon deleted exon 3 and created a null allele.
Labelled with:LacZ
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:Rasgrp3Gt(Pt1-ATG)1Vb/Rasgrp3+
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Roberts DM; Anderson AL; Hidaka M; Swetenburg RL; Patterson C; Stanford WL; Bautch VL, 2004 [PMID:15572660] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1128/MCB.24.24.10515-10528.2004] [ PMID:15572660] Roberts DM, Anderson AL, Hidaka M, Swetenburg RL, Patterson C, Stanford WL, Bautch VL 2004 A vascular gene trap screen defines RasGRP3 as an angiogenesis-regulated gene required for the endothelial response to phorbol esters. Mol Cell Biol (24):10515-28
Links:MGI:3525522 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE