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EMAGE:6005

Sulf1 sulfatase 1 ( MGI:2138563)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:6005 EMAGE:6005 EMAGE:6005
Fig 1B. [doi:10.1002/dvdy.21423] Copyright: This image is from Ratzka A; Kalus I; Moser M; Dierks T; Mundlos S; Vortkamp A, "Redundant function of the heparan sulfate 6-O-endosulfatases Sulf1 and Sulf2 during skeletal development." Dev Dyn 2008; 237: 339-53. Reprinted with permission of Wiley-Liss Inc. [PMID:18213582] Fig 1H. [doi:10.1002/dvdy.21423] Copyright: This image is from Ratzka A; Kalus I; Moser M; Dierks T; Mundlos S; Vortkamp A, "Redundant function of the heparan sulfate 6-O-endosulfatases Sulf1 and Sulf2 during skeletal development." Dev Dyn 2008; 237: 339-53. Reprinted with permission of Wiley-Liss Inc. [PMID:18213582] Fig 1K. [doi:10.1002/dvdy.21423] Copyright: This image is from Ratzka A; Kalus I; Moser M; Dierks T; Mundlos S; Vortkamp A, "Redundant function of the heparan sulfate 6-O-endosulfatases Sulf1 and Sulf2 during skeletal development." Dev Dyn 2008; 237: 339-53. Reprinted with permission of Wiley-Liss Inc. [PMID:18213582]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: aer, apical ectodermal ridge; dme, distal mesenchym; ect, ectoderm; fl, forelimb; fp, floor plate; hl, hindlimb; md, mandibular primordia; mx, maxillary primordia; oe, oral ectoderm; np, nasal placodes.
Expression Pattern Description
Spatial Annotation:
EMAGE:6005Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6005_wholemount__moderate.wlz
6005_wholemount__weak.wlz
6005_wholemount__possible.wlz
6005_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6005_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
forelimb bud apical ectodermal ridge
detected detected
telencephalon
detected detected
olfactory pit
detected detected
regionalExpressed in nasal placode
tail
detected detected
regional
trunk somite
detected detected
central nervous system
detected detected
regionalExpressed in floor plate
1st branchial arch mandibular component mesenchyme
detected detected
regionalExpressed in the distal mesenchyme of the mandibular primordia
oral region epithelium
detected detected
Expressed in oral ectoderm
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Sulf1 probeB
Entity Detected:Sulf1, sulfatase 1 ( MGI:2138563)
Sequence:sense strand is shown

>Sulf1 probeB
GACAGTTATGGGATGGATGGGAAGGTTAGTCAGTCCAATATTACTTCAGACACCAGCTGGCAAGGCCTGG
AGGAGTTATCCGGTGCAAGCAACATCGATGAGTACAGGTCTAACCCTAGACTAAGTCTGGAGGACTGGAC
TAACTACCTGAGGGCTGTCTACAGAGCCTTTGCACTGCTGAACAGTCACCCTGATCCAAACAAAGCAAAT
GGGACTCCAACCACACAAGGTGGTGACTTCCTGGTCACCTCTGCTGAGCGCTTGGTGCCAGCAGAGATGG
CTTCTGCAGAATCAGGTGAAGACCCAAGTCATGTGGTTGGGGAAACACCTCCTTTGACCTTGCCAGTCAA
CCTCCAAACCCTGCATCTGAACAGACCAACGTTAAGTCCAGAGAGAAAACTTGAATGGGATAATGACATT
CCAGAAGTGAATCATTTGAATTCTGAACACTGGAGAAAAACTGAGAAGCAGATAGGATGGGAGGAGCTGC
ATCATCCTGAAGGTGACGTCGTCAGTGGCAATGGTATGACAGAGCTGCTGCCCCAGTCTCATCTTGGGCA
TCAGCTCACCAGTCAGCACCAACAAAAATGTTCCCAGGATGTGGAGACAGAGAAGGATGCTTTTGAAGAT
CAATTGCGTCCTCTTGTCCACTCTGACAGAACTCCGGTTCATCGGGTGTTCGATGTGTCCCGTGTGGAGC
AGCCTCTTCACTCCAGCCACACGGAGAGAATGTTGGCCAAGATGGGGCAGAGTGATACAAAGGAGTGGCT
ACCAAATCTCGAAGGCAGTGCCTCCGCTCCAGTCATCTCTGCTTAGATTAGATGTTGCCTAACATAATAT
TTCTTTCCATGTTTTGATTAGTAAACTAGTGGTGGCAATCATGACTGCCAACATTCTGAGCCCCCACCTC
CACCCCCCTCCCTGGGCACACTCATGTGCACAGCAGACACTCATGTGCACGTGTGTGGGCAATGCTCACA
CAGACTCATTGTAACTTGCTATTCTACCAAGAAGGGAGAGGTGGGGGCACATTTTCTGTTTTGATGCTTT
GTTTTTGGTACTAAAACAGTATTATCTTTTGAATATAGTAGGGACATGAGTATATAAAATCTATCCAGTC
AAAATGGCTAGAATTGTGCCTTTGTAAGTTTTAAAAGTTTGATGCCCCTGGTGAGTCTGTCAACATATCT
TCTCTGCCTGCATAGTGAAGTTTGAATTTGTTTCTAACCACTGGAATTTTTCAACATCATCATCTTTGGT
TCAATGATTTTGCACTTTGAGTTTGGAATACTGTGTCTGCTTGGTTGGTAGTGTTATTATTTTTCTTTTA
AACAGGCTTATCAGAGTCACACACTTTGTCCTAGGAAGGACAAAGGAATAGACCCCCGGCAAGGACACAC
AGTATGGATCACATATTGTTTAGCGTACGCTTTTCCCAGGAACCATTGCATGTATTTTACCTCGACGTGC
TAAACTTGATTAGCAGAAAGGCATGGCTCACAATGTTGGTGGTAAGAAATAAATCCTGCGGGAATAAGCA
ATAAATAAAGCAAGAATGAAAAAAAAAAAAAAA
nt 3051 - nt 4623 of BC034547.1
Notes:The Sulf1 probe used in this study by Ratzka et al., 2008 [PMID:18213582] was "PCR amplified from IMAGE clone 4500954 with primers Sulf1-3utr-F: 5'-GACAGTTATGGGATGGATG and T7: 5'-TAATACGACTCACTATAGGGA". The IMAGE clone was obtained from RZPD (Berlin). Editors Note: the Sulf1-3utr-F primer sequence is found just prior to the 3'UTR and the T7 primer is found in the pCMV-SPORT6 vector adjacent to the multiple cloning site. It is assumed the T7 primer is at the 3' end of the insert in order for PCR amplification to have worked.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Ratzka A; Kalus I; Moser M; Dierks T; Mundlos S; Vortkamp A, 2008 [PMID:18213582] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.21423] [ PMID:18213582] Ratzka A, Kalus I, Moser M, Dierks T, Mundlos S, Vortkamp A 2008 Redundant function of the heparan sulfate 6-O-endosulfatases Sulf1 and Sulf2 during skeletal development. Dev Dyn (237):339-53
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE