EMAGE mouse gene expression spatial database

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EMAGE:6006

Sulf2 sulfatase 2 ( MGI:1919293)
TS16 (10.5 dpc)
in situ hybridisation

Data Images


Fig 1E. [doi:10.1002/dvdy.21423] Copyright: This image is from Ratzka A; Kalus I; Moser M; Dierks T; Mundlos S; Vortkamp A, "Redundant function of the heparan sulfate 6-O-endosulfatases Sulf1 and Sulf2 during skeletal development." Dev Dyn 2008; 237: 339-53. Reprinted with permission of Wiley-Liss Inc. [PMID:18213582]	Fig 1I. [doi:10.1002/dvdy.21423] Copyright: This image is from Ratzka A; Kalus I; Moser M; Dierks T; Mundlos S; Vortkamp A, "Redundant function of the heparan sulfate 6-O-endosulfatases Sulf1 and Sulf2 during skeletal development." Dev Dyn 2008; 237: 339-53. Reprinted with permission of Wiley-Liss Inc. [PMID:18213582]	Fig 1Q. [doi:10.1002/dvdy.21423] Copyright: This image is from Ratzka A; Kalus I; Moser M; Dierks T; Mundlos S; Vortkamp A, "Redundant function of the heparan sulfate 6-O-endosulfatases Sulf1 and Sulf2 during skeletal development." Dev Dyn 2008; 237: 339-53. Reprinted with permission of Wiley-Liss Inc. [PMID:18213582]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Note: ect, ectoderm; fl, forelimb; fp, floor plate; hl, hindlimb.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6006_wholemount__moderate.wlz

6006_wholemount__strong.wlz

6006_wholemount__possible.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6006_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon

detected

Broad expression, with partially overlapping regions to Sulf1 in the telencephalic vesicle, the nasal placodes, the somites, the tip of the tail, and the floor plate.

trunk somite

detected

Broad expression, with partially overlapping regions to Sulf1 in the telencephalic vesicle, the nasal placodes, the somites, the tip of the tail, and the floor plate.

olfactory pit

detected

Broad expression, with partially overlapping regions to Sulf1 in the telencephalic vesicle, the nasal placodes, the somites, the tip of the tail, and the floor plate.

neural tube floor plate

detected

Broad expression, with partially overlapping regions to Sulf1 in the telencephalic vesicle, the nasal placodes, the somites, the tip of the tail, and the floor plate.

forelimb bud apical ectodermal ridge

not detected

homogeneous

forelimb bud mesenchyme

strong

hindlimb bud mesenchyme

strong

1st branchial arch mandibular component mesenchyme

strong

1st branchial arch maxillary component mesenchyme

strong

1st branchial arch mandibular component ectoderm

not detected

homogeneous

1st branchial arch maxillary component ectoderm

not detected

homogeneous

1st branchial groove ectoderm

not detected

homogeneous

1st branchial membrane ectoderm

not detected

homogeneous

1st branchial arch maxillary-mandibular groove ectoderm

not detected

homogeneous

central nervous system

detected

regional

Expressed in floor plate

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Sulf2 probeA
Entity Detected:	Sulf2, sulfatase 2 ( MGI:1919293)
Sequence:	sense strand is shown >Sulf2 probeA CCAGAAATGAAGAGACCTTCTTCCAAATCACTGGGACAGCTATGGGAAGGTTGGGAAGGCTAAGCGGCCA TAGAGAGAGGAACCTCCAAAACCAGGGGCCTCGTGTGGCTGCCCAGGCCATGCAAAAAACACCCGATTCC CAGAAGATGAATGTTGGAACTGGGAGACCTGACAGAAGGCAGGGCCTGCTCTTGGGACAGGAAATCCTGG AGGACAGCGCCTGGACTTTCCGATGCTCAGTTTCTTTGCCCTGCTTTGCTCTGGATCAAACCTCACTGGC TGCTCTGGGATGCGTGCTCACACCTGGAGTCTCTGCTCACCCTTTCAGAGGCTCACAAAGACAAAGGAAC TAATTTCCATGGACACTTCCTCCAGAGATGGAAATTGCTGGGATTCGCCCACTCCTCCCCTGCACCCCTC CCCCAGTCATCTAGGGAAGCAAGCTTGTTTTAACCTTCTTACTCTTTGGAGAAAGCACGGACATCCCAGG TGCTGTCAACCTCACAGTCTTGACAAAGTCTATAGCACAAAACAGTACCATTCACCAGGCTGGTTGACCT GGCTGGCTCAGAAGCTGCCTTCACCACATACATGACCGCTCACACGTAACCAACACAGGGAATTGTAGGG GAATCTCACTAATATGAAATCCCGCTTTTCAAGAGTCGCGGTGTCAATAAACGCTGTGGCTAGGATCAAG GATAATCCCTTGAGCTTTCAGACATTTATTCCTGCCCGGGATTCGTTCCTTTGTTATCCATCCCAGAACT GATGTTTTTCTAAGGTACCGAAACCCCAAGTTGATGTGTGTCCTGTGTTTTAATGACATTGTATTTGTAA AGTTTTGTAGTATAAGTACCATCTTACAGTGTTCCTGCCCCCAGCCAATGTCTAGCTATTGGTATGAAAA AAAAATCTTTGAATTTTTGTAAAAAAAAAAAAAAAA
	nt 2658 - nt 3603 of AY101177.1
Notes:	The Sulf2 probe used in this study by Ratzka et al., 2008 [PMID:18213582] was "PCR amplified from IMAGE clone 3155559 with primers Sulf2-3utr-F: 5'-CCAGAAATGAAGAGACCTTC and Sp6: CTATTTAGGTGACACTATAG.". The IMAGE clone was obtained from RZPD (Berlin). Editors Note: the Sulf2-3utr-F primer sequence is found near the 3'end of the ORF and the Sp6 primer is found in the pCMV-SPORT6 vector adjacent to the multiple cloning site. It is assumed the Sp6 primer is at the 3' end of the insert in order for PCR amplification to have worked.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	10.5 dpc
Theiler Stage:	TS16
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:

4% paraformaldehyde

General Information

Authors:	Ratzka A; Kalus I; Moser M; Dierks T; Mundlos S; Vortkamp A, 2008 [PMID:18213582] . Indexed and spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1002/dvdy.21423] [ PMID:18213582] Ratzka A, Kalus I, Moser M, Dierks T, Mundlos S, Vortkamp A 2008 Redundant function of the heparan sulfate 6-O-endosulfatases Sulf1 and Sulf2 during skeletal development. Dev Dyn (237):339-53
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source