Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:6009

Chd2 chromodomain helicase DNA binding protein 2 ( MGI:2448567)
TS18 (10.5 dpc)
in situ reporter

Data Images
EMAGE:6009
Figure 3A E10.5. [doi:10.1002/ajmg.a.32178] Kulkarni S; Nagarajan P; Wall J; Donovan DJ; Donell RL; Ligon AH; Venkatachalam S; Quade BJ, "Disruption of chromodomain helicase DNA binding protein 2 (CHD2) causes scoliosis." Am J Med Genet A 2008 May 1;146A(9):1117-27. [PMID:18386809]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6009Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6009_wholemount__notDetected.wlz
6009_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6009_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
bulbus cordis
detected detected
common atrial chamber
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ reporter
Entity Detected:Chd2, chromodomain helicase DNA binding protein 2 ( MGI:2448567)
Notes:Chd2 expression was assayed in this study by Kulkarni et al., 2008 [PMID:18386809] in embryos heterozygous for a trapped allele that was obtained from the BayGenomics genetrap embryonic stem cell (ES) cell resource (Stryke et al., 2003 [PMID:12520002] - mutant cell line: RRB046). The genetrap vector present in the Chd2-targeted ES cell clone contains a promoter-less beta-galactosidase-neomycin fusion gene that allows expression analysis of the trapped Chd2 gene. Sequencing indicated that the gene trap was integrated within intron 27 (1,563 base pairs from the beginning of the intron) of Chd2.
Labelled with:LacZ
Specimen
Organism:mouse
Strain:involves: 129P2/OlaHsd * C57BL/6
Age:10.5 dpc
Theiler Stage:TS18
Mutations:Chd2Gt(RRBO46)Byg/Chd2+
Preparation:wholemount
Procedures
Fixation:1% paraformaldehyde
General Information
Authors:Kulkarni S; Nagarajan P; Wall J; Donovan DJ; Donell RL; Ligon AH; Venkatachalam S; Quade BJ, 2008 [PMID:18386809] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/ajmg.a.32178] [ PMID:18386809] Kulkarni S, Nagarajan P, Wall J, Donovan DJ, Donell RL, Ligon AH, Venkatachalam S, Quade BJ 2008 Disruption of chromodomain helicase DNA binding protein 2 (CHD2) causes scoliosis. Am J Med Genet A (146A):1117-27
 [ doi:10.1093/nar/gkg064] [ PMID:12520002] Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE 2003 BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. Nucleic Acids Res (31):278-81
Links:MGI:3800602 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE