EMAGE mouse gene expression spatial database

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EMAGE:6016

Ppp2r4 protein phosphatase 2A, regulatory subunit B (PR 53) ( MGI:1346006)
TS17 (10.5 dpc)
in situ hybridisation

Data Images


Raw signal output, as movie.	All sections along the X-axis, as movie.	All sections along the Y-axis, as movie.	All sections along the Z-axis, as movie.	Photograph prior to 3D imaging.

Photograph prior to 3D imaging.	Sample morphology following 3D imaging, as movie

View 3D opt image:
Download 3D images in woolz format
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

This is a traditionally produced wholemount in situ hybridisation stained using NBT + BCIP. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured red.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6016_wholemount__moderate.wlz

6016_wholemount__weak.wlz

6016_wholemount__notDetected.wlz

6016_wholemount__strong.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6016_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	PPP2R4_T7
Entity Detected:	Ppp2r4, protein phosphatase 2A, regulatory subunit B (PR 53) ( MGI:1346006)
Sequence:	sense strand is shown >PPP2R4_T7 TCTGGTGGCCGAGTAAGAAGAGAGGGGCCTGGACGTGGTCCCCGGCCCTGCCCATCCCTGTGCTCCTGCT TCCTCCCACGGTCAGGCCCATGTGGAGGAGACAAGGACACCCTGCTCTTGTCATTGCTCCCCCTTTCCTC CCCATGATGTAATGGTTCCCTCATTAAGAGCAGGAGTTCCGTGCCTCCAGGGCCGGCTGGTGGGTTCCCC CGCTAGCTGTGTTCCACTGGGTATCTGCTATGGAGCTGAAGGGTGAGGCTGTCATCAGCTGTTGGGAGGG CTGGGGTTGGGGGAGGTTGGTTTGTAGGGGCCATGGATGATGAGGCTGGTCTTCTCAAGGTACCATCCTA GGCCCTGGGGGACCTGTGTCCATCCTCATGCTGGCTTTGTTCCCTGGCCCTTGGCTTTGCTAGGTTTCCT GACTGTTAACATAACACTCCCCTTCCTGGGGATAGCCTAGCTATGGAAGCTGCCCTGGGCTCCTGGGAAA GCCCATTGCTTGGGTGAGATC
	nt 27151329 - nt 27151839 of NW_001030686.1
Notes:	This probe was transcribed from template produced by a PCR of whole genomic DNA using primers incorporating T3 and T7 promotors. The antisense probe was transcribed using the T7 polymerase. T3 5'-3' AATTAACCCTCACTAAAGGGTCTGGTGGCCGAGTAAGAA T7 5'-3' TAATACGACTCACTATAGGAGATCTCACCCAAGCAATGG Product size: 512 probe extends within above nt sequence (not exact co-ordinates)
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD-1
Age:	10.5 dpc
Theiler Stage:	TS17
Mutations:	none (wild-type)
Preparation:	opt

Procedures

Fixation:	4% paraformaldehyde
Embedding:	agarose
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Jacqueline Ramsay, David R Fitzpatrick
Principal investigator:	David R FitzPatrick, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Submitted by:	Jacqueline Ramsay, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Experiment type:	screen
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source