EMAGE mouse gene expression spatial database

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EMAGE:6022

Mkl2 MKL/myocardin-like 2 ( MGI:3050795)
TS17 (10.5 dpc)
in situ reporter

Data Images


Fig 3D. [doi:10.1073/pnas.0507346102] Copyright: This image is from Oh J; Richardson JA; Olson EN, Proc Natl Acad Sci U S A 2005 Oct 18;102(42):15122-7. [PMID:16204380] Copyright 2005 National Academy of Sciences, U.S.A.	Fig 3E. [doi:10.1073/pnas.0507346102] Copyright: This image is from Oh J; Richardson JA; Olson EN, Proc Natl Acad Sci U S A 2005 Oct 18;102(42):15122-7. [PMID:16204380] Copyright 2005 National Academy of Sciences, U.S.A.	Fig 3F. [doi:10.1073/pnas.0507346102] Copyright: This image is from Oh J; Richardson JA; Olson EN, Proc Natl Acad Sci U S A 2005 Oct 18;102(42):15122-7. [PMID:16204380] Copyright 2005 National Academy of Sciences, U.S.A.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Note this is a homozygous mutant embryo. The authors state "at E12.5 and earlier, homozygous mutants appeared outwardly normal and were present approximately at Mendelian ratios". E and F show higher magnification images of the left and right sides of the branchial region of the embryo in D. Image annotation: ba1, first branchial arch. Branchial arch arteries are numbered.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6022_wholemount__moderate.wlz

6022_wholemount__weak.wlz

6022_wholemount__possible.wlz

6022_wholemount__notDetected.wlz

6022_wholemount__strong.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6022_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

neural tube

detected

regional

Expression of lacZ was observed along the ventral neural tube.

rhombomere 03

detected

rhombomere 05

detected

ear

detected

Expression was also detected in the otic vesicle.

heart

detected

dorsal aorta

detected

1st arch artery

detected

2nd arch artery

detected

3rd arch artery

detected

1st branchial arch

detected

4th arch artery

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ reporter
Entity Detected:	Mkl2, MKL/myocardin-like 2 ( MGI:3050795)
Notes:	Mkl2 (MRTF-B) expression in this study by Oh et al., 2005 [PMID:16204380] , was assayed in embryos homozygous for a targeted allele where the targeting construct was designed to delete exon 8 by the introduction of a lacZ and neomycin resistance cassette. Deletion of exon 8 results in a truncated protein containing only the first 270 amino acids of the protein and lacking the SRF-binding, dimerization, and transcription activation domains. The lacZ reporter integrated into the targeted MRTF-B allele was expressed in a pattern corresponding to that of the wild-type gene.
Labelled with:	LacZ

Specimen

Organism:	mouse
Strain:	involves: 129S/SvEv * C57BL/6
Age:	10.5 dpc
Theiler Stage:	TS17
Mutations:	Mkl2^tm1Eno/Mkl2^tm1Eno
Preparation:	wholemount

Procedures

General Information

Authors:	Oh J; Richardson JA; Olson EN, 2005 [PMID:16204380] , Indexed and Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1073/pnas.0507346102] [ PMID:16204380] Oh J, Richardson JA, Olson EN 2005 Requirement of myocardin-related transcription factor-B for remodeling of branchial arch arteries and smooth muscle differentiation. Proc Natl Acad Sci U S A (102):15122-7
Links:	MGI:3608959 allele description
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source