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EMAGE:6061

Itsn1 intersectin 1 (SH3 domain protein 1A) ( MGI:1338069)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:6061
e9.5 WISH clone#218, [doi:10.1038/nature01270] Gitton et al., 2002 [PMID:12466855] Nature 2002 Dec 5;420(6915):586-90. The data image can be viewed at MGI

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6061Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6061_wholemount__moderate.wlz
6061_wholemount__weak.wlz
6061_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6061_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
The expression was widespread.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:293114
Entity Detected:Itsn1, intersectin 1 (SH3 domain protein 1A) ( MGI:1338069)
Sequence:sense strand is shown

>MGI:293114
GGAGATTTAACCTTTCAGCAAGGGGATGTGATTGTGGTTACCAAGAAAGATGGTGACTGGTGGACGGGAA
CGGTGGGCGACAAGTCCGGAGTCTTCCCTTCTAACTATGTGAGGCTTAAAGATTCAGAGGGCTCTGGAAC
TGCTGGGAAAACAGGGAGTTTAGGAAAAAAACCTGAAATTGCCCAGGTTATTGCTTCCTACGCTGCTACT
GGTCCCGAACAACTCACCCTGGCTCCTGGGCAGCTGATTCTGATCCGGAAAAAGAACCCAGGTGGATGGT
GGGAAGGAGAACTGCAAGCTCGAGGGAAAAAGCGCCAGATAGGGTGGTTTCCAGCAAATTATGTCAAACT
TCTAAGCCCCGGAACAAGCAAAATCACCCCAACTGAGCTACCCAAGACCGCAGTGCAGCCAGCAGTGTGC
CAGGTGATCGGGATGTACGATTACACCGGCCAGAACGATGACGAACTAGCCTTCAGCAAAGGCCAGATCA
TCAACGTCCTCAACAAGGAGGACCCGGACTGGTGGAAAGAAGAAGTC
Notes:The Itsn1 probe used in this study by Gitton et al., 2002 [PMID:12466855] is described as "synthesised by in vitro transcription from 1-2 ug of PCR-amplified cDNAs (insert size range of 500 - 1,500 bp)." Editors note: The clone used to derive this probe is specified on the project website as clone#218 (IMAGp998H191146).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Gitton Y; Dahmane N; Baik S; Ruiz I Altaba A; Neidhardt L; Scholze M; Herrmann BG; Kahlem P; Benkahla A; Schrinner S; Yildirimman R; Herwig R; Lehrach H; Yaspo ML, 2002 [PMID:12466855] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1038/nature01270] [ PMID:12466855] Gitton Y, Dahmane N, Baik S, Ruiz i Altaba A, Neidhardt L, Scholze M, Herrmann BG, Kahlem P, Benkahla A, Schrinner S, Yildirimman R, Herwig R, Lehrach H, Yaspo ML 2002 A gene expression map of human chromosome 21 orthologues in the mouse. Nature (420):586-90
Links:MGI:2674578 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI