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EMAGE:6064

Lss lanosterol synthase ( MGI:1336155)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:6064
e9.5 WISH clone#50, [doi:10.1038/nature01270] Gitton et al., 2002 [PMID:12466855] Nature 2002 Dec 5;420(6915):586-90. The data image can be viewed at MGI

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6064Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6064_wholemount__moderate.wlz
6064_wholemount__weak.wlz
6064_wholemount__possible.wlz
6064_wholemount__notDetected.wlz
6064_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6064_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
The expression was widespread.
branchial arch
detected detected
head mesenchyme
detected detected
trunk mesenchyme
detected detected
trunk somite
detected detected
tail paraxial mesenchyme
detected detected
trunk paraxial mesenchyme
detected detected
tail
detected detected
future brain
detected detected
future spinal cord
detected detected
limb
detected detected
nose
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1010478
Entity Detected:Lss, lanosterol synthase ( MGI:1336155)
Sequence:sense strand is shown

>MGI:1010478
GCTGGCAAGCGGTGGCAGAGAGTAGTGCTGCCATGACATAGGGCACGTGTCTGCGGCGTCGGGGGGGACC
CTATAAAACTGAGCCCGCCACCGATCTCACCCGCTGGCGGCTCCAGAATGAGTTGGGTCGGCAGAGATGG
ACTTATTATCAAGCGGAGGACGACCCTGGTCGAGAGCAGACCGGGCTAGAGGCCCACTCTTTGGGACTGG
ACACAAGAAGTTATTTCACGGACTTACCCAAAGCGCAAACAGCCCATGAGGGGGCCCTGAATGGAGTAAC
CTTTTATGCCAAGCTGCAGGCTGAGGATGGACACTGGGCTGGTGATTATGGTGGTCCGCTCTTCCTCTTG
CCAGGTCTCCTGATTACCTGTCACATATCACACATCTCTCTGCCAGCTGGATACAGAGAGGAGATGGTGC
GGTACCTGCGGTCAGTGCAGCTCCCTGATGGCGGGCTGGGCTTGCACATCTGAGACCAGTCCCCAGTGTT
TGGGACTGCCCTGAAC
Notes:The Lss probe used in this study by Gitton et al., 2002 [PMID:12466855] is described as "synthesised by in vitro transcription from 1-2 ug of PCR-amplified cDNAs (insert size range of 500 - 1,500 bp)." Editors note: The clone used to derive this probe is specified on the project website as clone#50 (IMAGp998F115745).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Gitton Y; Dahmane N; Baik S; Ruiz I Altaba A; Neidhardt L; Scholze M; Herrmann BG; Kahlem P; Benkahla A; Schrinner S; Yildirimman R; Herwig R; Lehrach H; Yaspo ML, 2002 [PMID:12466855] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1038/nature01270] [ PMID:12466855] Gitton Y, Dahmane N, Baik S, Ruiz i Altaba A, Neidhardt L, Scholze M, Herrmann BG, Kahlem P, Benkahla A, Schrinner S, Yildirimman R, Herwig R, Lehrach H, Yaspo ML 2002 A gene expression map of human chromosome 21 orthologues in the mouse. Nature (420):586-90
Links:MGI:2674662 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI