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EMAGE:6080

Slc19a1 solute carrier family 19 (sodium/hydrogen exchanger), member 1 ( MGI:103182)
TS15 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:6080
e9.5 WISH clone#580, [doi:10.1038/nature01270] Gitton et al., 2002 [PMID:12466855] Nature 2002 Dec 5;420(6915):586-90. The data image can be viewed at MGI

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6080Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6080_wholemount__moderate.wlz
6080_wholemount__weak.wlz
6080_wholemount__possible.wlz
6080_wholemount__notDetected.wlz
6080_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6080_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
The expression was widespread.
branchial arch
detected detected
head mesenchyme
detected detected
trunk mesenchyme
detected detected
trunk somite
detected detected
trunk paraxial mesenchyme
detected detected
tail paraxial mesenchyme
detected detected
limb
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2673791
Entity Detected:Slc19a1, solute carrier family 19 (sodium/hydrogen exchanger), member 1 ( MGI:103182)
Sequence:sense strand is shown

>MGI:2673791
TTTGTAGTTCTCCATTTTAATGTCAAGTTAAAAAAGAAATCAGAAAACCATTTAAACAAGCACCTCCGAT
AGTGGAAGGATGTATGGCAGCATAGCCAGGAGCCTAGGGCAGGCAACTGGCACCCTGCAGGCTCAGCGGA
CCTTGGCTGGCATCTGGGCTACCTCACTAGCCCTAAGGGGCAGACATGATTTGCCCAGAGCTTCCCAGAG
CAAAAAAGGCAGAATGGCCTGGTGGGCATAGTTGAGCCCGACACAGGTTCTTCTTGGCAAGCAATAGACA
ACCCAGACTCGCAGTGGCCTGGCACGTGGTTCACAAAGCCAGGGAGCTGCCCTTGAGTGTCGGTAGCAGG
CTACAGCCATTGCTAAAGCATGAAGTAGGCTGGGTTTAGGGTCCTCAGAAGGATAAGCTAACAGGTGGCC
CCTTAAGGAGGGTTCTGTGGCCTAACCTAAAGGCTCCTAAAANGCAACANCGGGGGCCCCCTGTATGTCT
TGGACTCAAGGCAACATCGACTGGGAACACTGTGTGTACCATCANGCCCAACCTAGTTCAGGTGGAATAC
AAACAAGAAGGGCTACCTCTGAATCAAGAATCCCCGCCCTGAACTTGGATGAAACTAAGAAAGTCA
Notes:The Slc19a1 probe used in this study by Gitton et al., 2002 [PMID:12466855] is described as "synthesised by in vitro transcription from 1-2 ug of PCR-amplified cDNAs (insert size range of 500 - 1,500 bp)." Editors note: The clone used to derive this probe is specified on the project website as clone#580 (IMAGp998G213873).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:10.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Gitton Y; Dahmane N; Baik S; Ruiz I Altaba A; Neidhardt L; Scholze M; Herrmann BG; Kahlem P; Benkahla A; Schrinner S; Yildirimman R; Herwig R; Lehrach H; Yaspo ML, 2002 [PMID:12466855] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1038/nature01270] [ PMID:12466855] Gitton Y, Dahmane N, Baik S, Ruiz i Altaba A, Neidhardt L, Scholze M, Herrmann BG, Kahlem P, Benkahla A, Schrinner S, Yildirimman R, Herwig R, Lehrach H, Yaspo ML 2002 A gene expression map of human chromosome 21 orthologues in the mouse. Nature (420):586-90
Links:MGI:2674646 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI