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EMAGE:6103

Sulf1 sulfatase 1 ( MGI:2138563)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:6103 EMAGE:6103 EMAGE:6103 EMAGE:6103 EMAGE:6103
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:6103 EMAGE:6103
Photograph prior to 3D imaging. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: three stars
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured blue. The sample has sustained considerable damage between the wholemount images and OPT scanning, this has caused seperation of the mandibular and maxilary components of the 1st branchial arch.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Sulf1_probeC
Entity Detected:Sulf1, sulfatase 1 ( MGI:2138563)
Sequence:sense strand is shown

>Sulf1_probeC
ACCCTGCATCTGAACAGACCAACGTTAAGTCCAGAGAGAAAACTTGAATGGGATAATGACATTCCAGAAG
TGAATCATTTGAATTCTGAACACTGGAGAAAAACTGAGAAGCAGATAGGATGGGAGGAGCTGCATCATCC
TGAAGGTGACGTCGTCAGTGGCAATGGTATGACAGAGCTGCTGCCCCAGTCTCATCTTGGGCATCAGCTC
ACCAGTCAGCACCAACAAAAATGTTCCCAGGATGTGGAGACAGAGAAGGATGCTTTTGAAGATCAATTGC
GTCCTCTTGTCCACTCTGACAGAACTCCGGTTCATCGGGTGTTCGATGTGTCCCGTGTGGAGCAGCCTCT
TCACTCCAGCCACACGGAGAGAATGTTGGCCAAGATGGGGCAGAGTGATACAAAGGAGTGGCTACCAAAT
CTCGAAGGCAGTGCCTCCGCTCCAGTCATCTCTGCTTAGATTAGATGTTGCCTAACATAATATTTCTTTC
CATGTTTTGATTAGTAAACTAGTGGTGGCAATCATGACTGCCAACATTCTGAGC
nt 12849252 - nt 12849795 of NC_000067.5
Notes:This probe was transcribed from template produced by a PCR of whole genomic DNA using primers incorporating T3 and T7 promotors. The probes were transcribed using the T7 polymerase. Primers: 5' AATTAACCCTCACTAAAGGACCCTGCATCTGAACAGACC 3' 5' TAATACGACTCACTATAGGGCTCAGAATGTTGGCAGTCA 3' Product length: 544bp
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Peter Branney, David R FitzPatrick
Principal investigator:David R FitzPatrick, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Submitted by:Peter Branney, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE