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EMAGE:6112

Egfr epidermal growth factor receptor ( MGI:95294)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:6112 EMAGE:6112 EMAGE:6112 EMAGE:6112 EMAGE:6112
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:6112 EMAGE:6112
Photograph prior to 3D imaging. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured blue.
Expression Pattern Description
Spatial Annotation:
EMAGE:6112Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6112_wholemount__moderate.wlz
6112_wholemount__weak.wlz
6112_wholemount__possible.wlz
6112_wholemount__notDetected.wlz
6112_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6112_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Egfr_probeB
Entity Detected:Egfr, epidermal growth factor receptor ( MGI:95294)
Sequence:sense strand is shown

>Egfr_probeB
CTGAGCAGGCCACACTTGTACAGCATTAAACCATGGCAGATACAATAGGATAAGCCACTTTGTTACTTAC
TGGGGCTGGGAGAAGAGGAATGACGGGGTAGAATTTTCCCTCAGACGTACTTTTTATATAAATATGTCCC
TGGCACCTAACACGCGCTAGTTTACCAGTGTTTTCTATTAGACTTCCTTCTATGTTTTCTGTTTCATTGT
TTTGAGTTGTAAATATGTGTTCCTGTCTTCATTTCATGAAGTAAACAAACAAACAAAAAACCCAGTATTA
AGTATTATCAAAGAACAACCATGATTCCACATTCGAACCCATTCAAACCATCAGTATTGTGACCAAAAGC
CTTTAACTAAGAAGGAGTAACCATGCAAAAATCCATAGAGGAATTTAACCCAAAATTTTAGTCTCAGCAT
TGTGTCTGCTGAGGTGTGTATATGAGACTACGAAAGTGAACTACTCTTCAAATCCACTTTGCCTTCACTC
CT
nt 16812360 - nt 16812851 of NC_000077.5
Notes:This probe was transcribed from template produced by a PCR of whole genomic DNA using primers incorporating T3 and T7 promotors. The probes were transcribed using the T7 polymerase. Primers: T3: 5' AATTAACCCTCACTAAAGGCTGAGCAGGCCACACTTGTA 3' T7: 5' TAATACGACTCACTATAGGAGGAGTGAAGGCAAAGTGGA 3' Product length: 492bp
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Peter Branney, David R Fitzpatrick
Principal investigator:David R FitzPatrick, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Submitted by:Peter Branney, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE