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EMAGE:6118

Fzd5 frizzled homolog 5 (Drosophila) ( MGI:108571)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:6118 EMAGE:6118 EMAGE:6118 EMAGE:6118 EMAGE:6118
3D reconstructed object showing signal. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: one star
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography using visible light. In the OPT images, the signal is visible as the brightest regions.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Fzd5 probeA
Entity Detected:Fzd5, frizzled homolog 5 (Drosophila) ( MGI:108571)
Sequence:sense strand is shown

>Fzd5 probeA
AGTGCCCAACTGCGCGGTGCCCTGCTACCAGCCGTCCTTCAGCCCGGACGAGCGCACATTCGCCACCTTC
TGGATTGGCCTGTGGTCTGTGCTGTGCTTCATCTCCACGTCCACCACCGTTGCCACCTTCCTCATTGACA
TGGAACGATTCCGCTACCCTGAGCGCCCCATCATCTTCTTGTCTGCGTGCTACCTGTGTGTGTCACTGGG
ATTCTTGGTGCGCCTGGTAG
nt 1513 - nt 1742 of NM_022721.3
Notes:The Fzd5 probe used in this study was obtained from U. Borello/C. Cossu. The full insert sequence of this clone has been verified by Summerhurst and Murphy (it spans nt 1513-1742 of the Fzd5 cDNA RefSeq NM_022721.3).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Kristen Summerhurst, Paula Murphy
Principal investigator:Paula Murphy, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Submitted by:Kristen Summerhurst, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Experiment type:non-screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE