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EMAGE:615

Fst follistatin ( MGI:95586)
TS12 (6 Somite no.)
in situ hybridisation

Data Images
EMAGE:615

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:615Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
615_wholemount_strong_3D_1.wlz
615_wholemount_moderate_3D_1.wlz
615_wholemount_weak_3D_1.wlz
615_wholemount_possible_3D_1.wlz
615_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:615_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future hindbrain neural ectoderm
strong strong
regionalRestricted to rhombomeres 1, 2, 4 and 6
trunk somite
detected detected
regionalDeclining expression towards posterior.
primitive streak
weak weak
regionalDeclining expression and no expression by 10 somite stage.
head somite
strong strong
regional
anterior pro-rhombomere
strong strong
Expressed in r1/2 neurectoderm
head paraxial mesenchyme
weak weak
regionalExpression in cranial paraxial mesoderm
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1202170
Entity Detected:Fst, follistatin ( MGI:95586)
Sequence:sense strand is shown

>MGI:1202170
TATCCAGTGTGGCGGCGGGAAAAAATGCCTATGGGATTCCAAGGTTGGCAGAGGTCGCTGCTCTCTCTGC
GATGAGCTGTGTCCTGACAGTAAGTCGGATGAGCCGGTCTGTGCCAGTGACAATGCCACATACGCCAGCG
AGTGTGCCATGAAGGAAGCTGCCTGCTCTTCTGGCGTGCTTCTTGAAGTGAAGCATTCTGGATCTTGCAA
CTCCATCTCGGAAGAAACGGAGGAAGAGGAGGAGGAGGAAGACCAGGACTACAGCTTTCCTATCTCTTCC
ATTCTAGAGTGGTAAACTCTCCACCAATGTTCAGTGTT
nt 738 - nt 1055 of Z29532.1
Notes:Probe used in this study is the HincII/EcoRV fragment originally described by Albano et al., 1994 [PMID:7600958] and shown in Fig 1 therein. Corresponds to the last 94 amino acids and the first 27 bases of the 3' UTR and detects both the long and short forms of follistatin. Clone linearised with EcoRV and transcribed using T3.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:6 Somite no.
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Principal investigator:Ruth Arkell, MRC Mammalian Genetics Unit, null, Harwell, UK OX11 ORD
Submitted by:Ruth Arkell, MRC Mammalian Genetics Unit, null, Harwell, UK OX11 ORD
Experiment type:non-screen
References:[ PMID:7600958] Albano RM, Arkell R, Beddington RS, Smith JC 1994 Expression of inhibin subunits and follistatin during postimplantation mouse development: decidual expression of activin and expression of follistatin in primitive streak, somites and hindbrain. Development (120):803-13
Links:MGI:1202170 same probe
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE