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EMAGE:6155

Wnt9a wingless-type MMTV integration site 9A ( MGI:2446084)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:6155 EMAGE:6155 EMAGE:6155 EMAGE:6155 EMAGE:6155
3D reconstructed object showing signal. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: two stars
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography using visible light. In the OPT images, the signal is visible as the brightest regions.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Wnt9a probeA
Entity Detected:Wnt9a, wingless-type MMTV integration site 9A ( MGI:2446084)
Sequence:sense strand is shown

>Wnt9a probeA
TGTCTTCTTCAGAGAGCCAAACCACTAGGAGAGGGCAGCCACTCCATCCCCAGGGTGCAGCCTCCGGCCC
TCTGACCCTGAGCACTTCCGTGGAGTATCAGTGACTTTTAAATTATTATTATTATTTAACTAATATAGTA
ATTATTATTTTCCCTGTCCCCTTACCTCCTCATCTGTGGCTTTTCCCTCTGAGCTGCCCCAGGCCCAGTC
AAGCCAGTCTGGGAGGCCTTGCCTTCCCTCTCTTCATCGTCCCCCCCTCCTTTGCTTGCTCTAGTTTGAA
ACCACTAAATCAAGGTCATGTTTGGAAAGAAATGAAGCGATAGAGCCTAGCCATGGCCCACAAGGCTTCT
GGAATCCACTCCCCAATTCTACCTTCCCCAGGGTCAGAGACCCTTGGTGGTCCCTGCTCTGTGCCCTGCA
GGTGCAGAGATCAGGTTGCTGGATATAGATGGGGGATTTGGGGGTCCTGGAGCCATGGGCACGGAGACAG
GGGCAGGGTCAGCTTTTTGATTGTCCATCTCAGGAAGCCATTCTTGCTCTGTGTCCCCAA
nt 1698 - nt 2247 of NM_139298.2
Notes:The Wnt9a probe template used in this study was generated by RT-PCR using the following primers: forward: 5'-GTCTTCTTCAGAGAGCCAAACCAC-3'; reverse: 5'-TGGGGACACAGAGCAAGAATGG-3', and this fragment was then cloned using a TOPO TA cloning kit (Invitrogen). Probe was transcribed from the entire insert.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Kristen Summerhurst, Paula Murphy
Principal investigator:Paula Murphy, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Submitted by:Kristen Summerhurst, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Experiment type:non-screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE