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EMAGE:6168

Pdgfc platelet-derived growth factor, C polypeptide ( MGI:1859631)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:6168 EMAGE:6168 EMAGE:6168 EMAGE:6168 EMAGE:6168
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:6168 EMAGE:6168
Photograph prior to 3D imaging. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured blue.
Expression Pattern Description
Spatial Annotation:
EMAGE:6168Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6168_wholemount__moderate.wlz
6168_wholemount__possible.wlz
6168_wholemount__notDetected.wlz
6168_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6168_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Pdgfc_probeB
Entity Detected:Pdgfc, platelet-derived growth factor, C polypeptide ( MGI:1859631)
Sequence:sense strand is shown

>Pdgfc_probeB
CCCTGCAGGACTTCAAGTGTCTTCTAAAAGACCAGAGGCACCAAGAGGAGTCAATCACAAAGCACTGCCT
TCTAGAGGAAGCCCAGACAATGGTCTTCTGACCACAGAAACAAATGAAATGAATGTAGATCGCTAGCAAA
CTCTGGAGTGACAGCATTTCTTTTCCACTGACAGAATGGTGTAGCTTAGTTGTCTTGATATGGGCAAGTG
ATGTCAGCACAAGAAAATGGTGAAAAACACACACTTGATTGTGAACAATGCAGAAATACTTGGATTTCTC
CAACCTGTTTGCATAGATAGACAGATGCTCTGTTTTCTACAAACTCAAAGCTTTTAGAGAGCAGCTATGT
TAATAGGAATTAAATGTGCCATGCTGAAAGGAAAGACTGAAGTTTTCAATGCTTGGCAACTTCTCCGCAA
TTTGGAGGAAAGGTGCGGTCATGGTTTGGAGAAAGCACACCTGCACAG
nt 81016670 - nt 81017137 of NC_000069.5
Notes:This probe was transcribed from template produced by a PCR of whole genomic DNA using primers incorporating T3 and T7 promotors. The probes were transcribed using the T7 polymerase. Primers: T3: 5' AATTAACCCTCACTAAAGGCCCTGCAGGACTTCAAGTGT 3' T7: 5' TAATACGACTCACTATAGGCTGTGCAGGTGTGCTTTCTC 3' Product length: 468bp
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Peter Branney, David R Fitzpatrick
Principal investigator:David R FitzPatrick, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Submitted by:Peter Branney, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE