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EMAGE:6171

Aldh7a1 aldehyde dehydrogenase family 7, member A1 ( MGI:108186)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:6171 EMAGE:6171 EMAGE:6171 EMAGE:6171 EMAGE:6171
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:6171 EMAGE:6171
Photograph prior to 3D imaging. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: one star
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured blue. The visible staining in this specimen is likely to be background rather than true signal and has been annotated accordingly in the text mapping.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo
not detected not detected
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:ALDH7A1_probeA
Entity Detected:Aldh7a1, aldehyde dehydrogenase family 7, member A1 ( MGI:108186)
Sequence:sense strand is shown

>ALDH7A1_probeA
TAGTGCAGTGCTGGAGATGGAATCCCAGGCATTGAGTGGCAAGCAGCCTACCTAACCAAGCTATATTCAC
AGCCCCTTTAATTTTTATAGTCAAATCTACTACAAATTTAATTATATAAAGGTATATCACCATATTATTT
AATATGTTAGTATTAGTGTCCCCCCTGCTTCTTCCCCACACTGTCAGTCCCCCAGATTTCTTACTATTGT
CCAAATCTTTCCACACCATGACGACTCATGAGGCCTACAATGTCCTATTCCCTAAGGGTTTCTCCAGGAG
CCCGATGTGGCAGGAGCAGGCCCAGTGGTGACTGCTACACAGAAGTACAGAGCAACGGCACCACAGGAGA
GCAGGGACCGCTGGGCCCAGCACAGCTTCCCAGTGAGACCTTAGCCTCTCAGCAGTGACCTTCC
nt 56685418 - nt 56685831 of NC_000084.5
Notes:This probe was transcribed from template produced by a PCR of whole genomic DNA using primers incorporating T3 and T7 promotors. The antisense probe was transcribed using the T7 polymerase. Primers: 5' AATTAACCCTCACTAAAGGGGAAGGTCACTGCTGAGAGG 3' 5' TAATACGACTCACTATAGGTAGTGCAGTGCTGGAGATGG 3' Product length: 414bp
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Peter Branney, David R FitzPatrick
Principal investigator:David R FitzPatrick, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Submitted by:Peter Branney, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE