EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:6207

Atp2b2 ATPase, Ca++ transporting, plasma membrane 2 ( MGI:105368)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012931. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012931_01	euxassay_012931_02	euxassay_012931_03	euxassay_012931_04

euxassay_012931_05	euxassay_012931_06	euxassay_012931_07	euxassay_012931_08	euxassay_012931_09

euxassay_012931_10	euxassay_012931_11	euxassay_012931_12	euxassay_012931_13	euxassay_012931_14

euxassay_012931_15	euxassay_012931_16	euxassay_012931_17	euxassay_012931_18	euxassay_012931_19

euxassay_012931_20	euxassay_012931_21	euxassay_012931_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6207_wholemount_strong.wlz

6207_wholemount_moderate.wlz

6207_wholemount_weak.wlz

6207_wholemount_possible.wlz

6207_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6207_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 17 weak expression: see section 10 11 12 13 14 15 16

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 07 moderate expression: see section 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 moderate expression: see section 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 17 18 moderate expression: see section 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 08 moderate expression: see section 18 19

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 07 moderate expression: see section 18 19

ventral grey horn

strong

regional

strong expression: see section 10 11 13 15 moderate expression: see section 12 14

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38476
Entity Detected:	Atp2b2, ATPase, Ca++ transporting, plasma membrane 2 ( MGI:105368)
Sequence:	sense strand is shown >T38476 TCTCACTGGCCTATTCTGTGAAGAAAATGATGAAGGACAACAACCTGGTACGCCACCTGGATGCCTGTGA GACCATGGGCAATGCCACAGCCATCTGCTCAGACAAGACAGGAACGCTGACCACCAACCGCATGACCGTG GTCCAGGCCTATGTCGGTGACGTCCACTACAAGGAGATCCCCGATCCCAGCTCCATCAATGCCAAGACGC TGGAGCTGCTGGTCAACGCCATTGCCATCAACAGCGCCTACACCACCAAGATCCTTCCCCCAGAAAAAGA GGGAGCCCTGCCCCGGCAGGTGGGCAACAAGACAGAGTGCGGCCTGCTGGGCTTTGTGCTGGACTTGAGG CAGGACTACGAGCCGGTGCGCAGCCAGATGCCAGAGGAGAAGCTGTATAAGGTGTACACCTTCAACTCCG TGCGCAAGTCCATGAGCACCGTCATCAAGATGCCCGACGAGAGCTTCCGCATGTACAGCAAGGGCGCCTC GGAGATTGTGCTCAAAAAGTGCTGCAAGATCCTCAGTGGGGCAGGGGAAGCCCGTGTCTTCCGGCCCCGA GACAGGGATGAGATGGTTAAGAAGGTGATCGAGCCCATGGCCTGTGACGGGCTCCGTACCATCTGCGTGG CCTATCGTGACTTCCCCAGCAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 150681. Forward Primer - name:150681_F_cDNA_Atp2b2, sequence:TCTCACTGGCCTATTCTGTGAA; Reverse Primer - name:150681_N_SP6_cDNA_Atp2b2, sequence:CTGCTGGGGAAGTCACGATAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6208 same embryo
	EMAGE:6210 same embryo
	EMAGE:6209 same embryo
	EurExpress:euxassay_012931 same experiment
	MGI:4823310 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source