EMAGE mouse gene expression spatial database

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EMAGE:6229

Fam65b family with sequence similarity 65, member B ( MGI:2444879)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013126. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013126_01	euxassay_013126_02	euxassay_013126_03	euxassay_013126_04

euxassay_013126_05	euxassay_013126_06	euxassay_013126_07	euxassay_013126_08	euxassay_013126_09

euxassay_013126_10	euxassay_013126_11	euxassay_013126_12	euxassay_013126_13	euxassay_013126_14

euxassay_013126_15	euxassay_013126_16	euxassay_013126_17	euxassay_013126_18	euxassay_013126_19

euxassay_013126_20	euxassay_013126_21	euxassay_013126_22	euxassay_013126_23	euxassay_013126_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6229_wholemount_strong.wlz

6229_wholemount_moderate.wlz

6229_wholemount_possible.wlz

6229_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6229_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 09 10 11 12 14 15 16 17

telencephalon mantle layer

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 14 15 16 17 21 22

midbrain mantle layer

weak

regional

weak expression: see section 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39435
Entity Detected:	Fam65b, family with sequence similarity 65, member B ( MGI:2444879)
Sequence:	sense strand is shown >T39435 GGAAGCCATACCAGAATTTCACAAAAAGCTCTCCTTGCTGGCCTTCTGGACCAAATGCTGCAGCCCCAGC GGGGTCTACCACAGCTCCGCTGCCCGCCTCATCAAGCAGCTGGAGGCCAGCTTCGCCAGAAGCATCAACA AAGACTATCCAGGACTGGCAGAGCCAGTGTTTAGAACCCTGGTGTCCCAAATCCTGGACCGAGCTGAACC TCTCCTTTCCTCCAGCCTCTCGTCGGAAGTCATCACTGTCTTCCAGTACTACAGCTTCTTCACCAGCCAC GGTGTGAGTGACCTGGAGACTTACCTGGGCCAGCTAACCAGGCAAGTCAACTTCCTGGTGTTGGAGCGGG AGCTTGCTAAGCACGCTGGTCCCCAGCAGGAGTTCACTGTGAACAGGAAACTGGCAAGGGAATGGAAGTC TCAGGGCTTTCCTTCCTTTCTCAACTTGTTTGCTGTGCTGTTTGATGCTGTGTTTTGTGGTGTTGCCATG GTTCAGACTCTGCAGTCACTGAGAGATGAGAAACTGCTGCAGACTATGAGCGACCTTGCACCCAGCAACC TCCCCGCTCAACAGGAAGTCCTCAGGACGCTGGCCCTGCTGCTCACCAGGGATGACAATGAGGTCAGCGA AGCTGTGACACTCTACTTGGCAGCAGCCTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 84101. Forward Primer - name:084101_F_cDNA_6330500D04Rik, sequence:GGAAGCCATACCAGAATTTCAC; Reverse Primer - name:084101_N_SP6_cDNA_6330500D04Rik, sequence:GAGGCTGCTGCCAAGTAGAGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6226 same embryo
	EMAGE:6231 same embryo
	EMAGE:6228 same embryo
	EMAGE:6230 same embryo
	EMAGE:6227 same embryo
	EurExpress:euxassay_013126 same experiment
	MGI:4824754 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source