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EMAGE:623

Bmp7 bone morphogenetic protein 7 ( MGI:103302)
TS10
in situ hybridisation

Data Images
EMAGE:623

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Anterior is to the left.
Expression Pattern Description
Spatial Annotation:
EMAGE:623Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
623_wholemount_strong_3D_1.wlz
623_wholemount_moderate_3D_1.wlz
623_wholemount_possible_3D_1.wlz
623_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:623_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
amniotic fold mesoderm
strong strong
homogeneous
node
strong strong
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:Bmp7 probeA
Entity Detected:Bmp7, bone morphogenetic protein 7 ( MGI:103302)
Sequence:sense strand is shown

>Bmp7 probeA
CGATGTCTCACCATCTAAGTCTCTCACTGCCCACCTTGGCGAGGAGCCAACAGACCAACCTCTCCTGAGC
CTTCCCCTCACCTCCCCAACCGGAAGCATGTAAGGGTTCCAGAAACCTGAGCGTGCAGGCAGCTGATGAG
CGCCCTTTCCTTCTGGCACGTGACGGACAAGATCCTACCAGCTACCACAGCAAACGCCTAAGAGCAGGAA
AAATGTCTGCCAGGAAAGTGTCCATTGGCCACATGGCCCCTGGCGCTCTGAGTCTTTGAGGAGTAATCGC
AAGCCTCGTTCAGCTGCAGCAGAAGGAAGGGCTTAGCCAGGGTGGGCGCTGGCGTCTGTGTTGAAGGGAA
ACCAAGCAGAAGCCACTGTAATGATATGTCACAATAAAACCCATGAATGAAAAAAAAAAAAAAAAAAAAA
AAAAAAAA
nt 1445 - nt 1872 of X56906.1
Notes:This probe covers the last 427 bases of the Bmp7 3'UTR (see Ozkaynak et al 1991 [PMID:1715687] and associated X56906.1 for sequence).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Theiler Stage:TS10
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Principal investigator:Ruth Arkell, MRC Mammalian Genetics Unit, null, Harwell, UK OX11 ORD
Submitted by:Ruth Arkell, MRC Mammalian Genetics Unit, null, Harwell, UK OX11 ORD
Experiment type:non-screen
References:[ doi:10.1016/0006-291X(91)91342-A] [ PMID:1715687] Ozkaynak E, Schnegelsberg PN, Oppermann H 1991 Murine osteogenic protein (OP-1): high levels of mRNA in kidney. Biochem Biophys Res Commun (179):116-23
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE