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EMAGE:6239

Gm6422 predicted gene 6422 ( MGI:3644358)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6239 EMAGE:6239 EMAGE:6239 EMAGE:6239 EMAGE:6239
"Pseudo-wholemount" of euxassay_016080. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016080_01 euxassay_016080_02 euxassay_016080_03 euxassay_016080_04
EMAGE:6239 EMAGE:6239 EMAGE:6239 EMAGE:6239 EMAGE:6239
euxassay_016080_05 euxassay_016080_06 euxassay_016080_07 euxassay_016080_08 euxassay_016080_09
EMAGE:6239 EMAGE:6239 EMAGE:6239 EMAGE:6239 EMAGE:6239
euxassay_016080_10 euxassay_016080_11 euxassay_016080_12 euxassay_016080_13 euxassay_016080_14
EMAGE:6239 EMAGE:6239 EMAGE:6239 EMAGE:6239 EMAGE:6239
euxassay_016080_15 euxassay_016080_16 euxassay_016080_17 euxassay_016080_18 euxassay_016080_19
EMAGE:6239 EMAGE:6239 EMAGE:6239 EMAGE:6239 EMAGE:6239
euxassay_016080_20 euxassay_016080_21 euxassay_016080_22 euxassay_016080_23 euxassay_016080_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6239Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6239_wholemount_strong.wlz
6239_wholemount_moderate.wlz
6239_wholemount_weak.wlz
6239_wholemount_possible.wlz
6239_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6239_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39871
Entity Detected:Gm6422, predicted gene 6422 ( MGI:3644358)
Sequence:sense strand is shown

>T39871
TTATCTTCAGTAGCCCAGGGAATGGAACAAGACGGGACCTCATCAGGAAAGCCTGGGGTAGTGTGACCAC
TGTCCAAGGCTACCCAATTCTCACGCTGTTTGCTCTGGGAATGCCAGCATTGGTAACTACTCAAGAAGAG
ATAGATGCGGAGTCTCAGAAGAATAATGATATCATTGAAGGGATCTTCTTGGACAGTTCTGAAAACCAAA
CACTGAAGATCATCTCAATGACCCAGTGGGCTGTGGCCTTCTGCCCCAGTGCCCTGTTCATACTCAAAGC
TGACGAGATGTTTATCAATCTACCAGGCTTGGTAGACTATCTTCTCAATCTGAAAGGTCACCTGGAAGGT
ATCTATTTAGGAAGAGTTATTCATCAGGATATACCTAACAGAGACCCCCATAGTCAAGAATTTGTCTCTC
TTAGTGAGTACCCCGAAAAATACTACCCAGACTACTGCAGTGGTGAGGCCTTTATACTGTCCCAAGATGT
GGCTCGGATGGTGTACGTGGTTTTGAATGAAGCACCTGTTATGGTGCCCGCCGATGTATTTGTAGGGATC
TGTGCTAAGTCCGTGGGCCTTATACCTATCCACAGTTCAAGGTTTTCTGGGGAAAGACACATCACATATA
ACAGATGCTGCTACAAGTTCATTTTTACATCTGCAGAAGTTACAGATGCTGAAACTTCCCTGGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 76517. Forward Primer - name:076517_F_cDNA_LOC383682, sequence:TTATCTTCAGTAGCCCAGGGAA; Reverse Primer - name:076517_N_SP6_cDNA_LOC383682, sequence:GCCAGGGAAGTTTCAGCATCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6237 same embryo
 EMAGE:6241 same embryo
 EMAGE:6236 same embryo
 EMAGE:6238 same embryo
 EMAGE:6240 same embryo
 EurExpress:euxassay_016080 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS