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EMAGE:624

Bmp7 bone morphogenetic protein 7 ( MGI:103302)
TS12
in situ hybridisation

Data Images
EMAGE:624

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:624Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
624_wholemount_strong_3D_1.wlz
624_wholemount_moderate_3D_1.wlz
624_wholemount_weak_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:624_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
surface ectoderm
weak weak
gut
strong strong
Expressed in gut endoerm in regions where the gut is closed
heart
strong strong
Expressed in myocardium
head paraxial mesenchyme
strong strong
Expression in cranial paraxial mesoderm
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:Bmp7 probeA
Entity Detected:Bmp7, bone morphogenetic protein 7 ( MGI:103302)
Sequence:sense strand is shown

>Bmp7 probeA
CGATGTCTCACCATCTAAGTCTCTCACTGCCCACCTTGGCGAGGAGCCAACAGACCAACCTCTCCTGAGC
CTTCCCCTCACCTCCCCAACCGGAAGCATGTAAGGGTTCCAGAAACCTGAGCGTGCAGGCAGCTGATGAG
CGCCCTTTCCTTCTGGCACGTGACGGACAAGATCCTACCAGCTACCACAGCAAACGCCTAAGAGCAGGAA
AAATGTCTGCCAGGAAAGTGTCCATTGGCCACATGGCCCCTGGCGCTCTGAGTCTTTGAGGAGTAATCGC
AAGCCTCGTTCAGCTGCAGCAGAAGGAAGGGCTTAGCCAGGGTGGGCGCTGGCGTCTGTGTTGAAGGGAA
ACCAAGCAGAAGCCACTGTAATGATATGTCACAATAAAACCCATGAATGAAAAAAAAAAAAAAAAAAAAA
AAAAAAAA
nt 1445 - nt 1872 of X56906.1
Notes:This probe covers the last 427 bases of the Bmp7 3'UTR (see Ozkaynak et al 1991 [PMID:1715687] and associated X56906.1 for sequence).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Principal investigator:Ruth Arkell, MRC Mammalian Genetics Unit, null, Harwell, UK OX11 ORD
Submitted by:Ruth Arkell, MRC Mammalian Genetics Unit, null, Harwell, UK OX11 ORD
Experiment type:non-screen
References:[ doi:10.1016/0006-291X(91)91342-A] [ PMID:1715687] Ozkaynak E, Schnegelsberg PN, Oppermann H 1991 Murine osteogenic protein (OP-1): high levels of mRNA in kidney. Biochem Biophys Res Commun (179):116-23
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE