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EMAGE:626

Shh sonic hedgehog ( MGI:98297)
TS15
in situ hybridisation

Data Images
EMAGE:626

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:626Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
626_wholemount_strong_3D_1.wlz
626_wholemount_moderate_3D_1.wlz
626_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:626_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
gut
moderate moderate
homogeneousExpressed in gut endoderm.
neural tube floor plate
strong strong
homogeneousExpressed along entire length of neural tube in floor plate.
notochord
strong strong
homogeneousExpressed along entire length of notochord
diencephalon floor plate
strong strong
Expressed in ventral diencephalon.
future midbrain floor plate
strong strong
Expressed along entire length of neural tube in floor plate.
rhombomere 01 floor plate
strong strong
Expressed along entire length of neural tube in floor plate.
rhombomere 02 floor plate
strong strong
Expressed along entire length of neural tube in floor plate.
rhombomere 03 floor plate
strong strong
Expressed along entire length of neural tube in floor plate.
rhombomere 04 floor plate
strong strong
Expressed along entire length of neural tube in floor plate.
rhombomere 05 floor plate
strong strong
Expressed along entire length of neural tube in floor plate.
rhombomere 06 floor plate
strong strong
Expressed along entire length of neural tube in floor plate.
rhombomere 07 floor plate
strong strong
Expressed along entire length of neural tube in floor plate.
rhombomere 08 floor plate
strong strong
Expressed along entire length of neural tube in floor plate.
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:19210
Entity Detected:Shh, sonic hedgehog ( MGI:98297)
Notes:Probe used in this study is the full length 2.6kb clone described by Echelard et al., 1993 [PMID:7916661] (includes 5' and 3' UTR and coding sequences).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Principal investigator:Ruth Arkell, MRC Mammalian Genetics Unit, null, Harwell, UK OX11 ORD
Submitted by:Ruth Arkell, MRC Mammalian Genetics Unit, null, Harwell, UK OX11 ORD
Experiment type:non-screen
References:[ doi:10.1016/0092-8674(93)90627-3] [ PMID:7916661] Echelard Y, Epstein DJ, St-Jacques B, Shen L, Mohler J, McMahon JA, McMahon AP 1993 Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity. Cell (75):1417-30
Links:MGI:19210 same probe
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE