EMAGE mouse gene expression spatial database

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EMAGE:6264

4732419C18Rik RIKEN cDNA 4732419C18 gene ( MGI:3045379)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016115. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016115_01	euxassay_016115_02	euxassay_016115_03	euxassay_016115_04

euxassay_016115_05	euxassay_016115_06	euxassay_016115_07	euxassay_016115_08	euxassay_016115_09

euxassay_016115_10	euxassay_016115_11	euxassay_016115_12	euxassay_016115_13	euxassay_016115_14

euxassay_016115_15	euxassay_016115_16	euxassay_016115_17	euxassay_016115_18	euxassay_016115_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6264_wholemount_strong.wlz

6264_wholemount_moderate.wlz

6264_wholemount_weak.wlz

6264_wholemount_possible.wlz

6264_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6264_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex mantle layer

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 17 18 19

telencephalon mantle layer

weak

regional

weak expression: see section 04 08 09

olfactory cortex mantle layer

weak

regional

weak expression: see section 07 08 12

pons mantle layer

moderate

regional

moderate expression: see section 09 weak expression: see section 08 14 15

facial vii ganglion

strong

single cell

strong expression: see section 03 moderate expression: see section 04 05 weak expression: see section 18

glossopharyngeal ix ganglion

moderate

single cell

moderate expression: see section 06 weak expression: see section 16

trigeminal v ganglion

strong

single cell

strong expression: see section 02 03 moderate expression: see section 04 05 06 weak expression: see section 15 16 17 18 19

vagus x ganglion

moderate

single cell

moderate expression: see section 07

vestibulocochlear viii ganglion

moderate

single cell

moderate expression: see section 05

ventral grey horn

moderate

regional

moderate expression: see section 09 10 14 weak expression: see section 11 12 13

dorsal root ganglion

moderate

single cell

moderate expression: see section 07 08 09 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40065
Entity Detected:	4732419C18Rik, RIKEN cDNA 4732419C18 gene ( MGI:3045379)
Sequence:	sense strand is shown >T40065 GAACAGGGAAGAAAATAGGGCTGGGCTTCCTCCCATCCAGGGAAGAGAGGAGAGAGGGAGGGAGGGAGAG AGAGAGAGAGAGGACGAGGAGGAGGTGGGGATTTGCCACCAGGGGAGAGTCTGGAAGATGCCATGGAAAA ACACCTGGAGCAAAGAAAGCCATGAAATGCTATTATTTCAGGGATTTTTGGCTGGGAGGTAGCTAGCTTA GTGTAGAGGATTAAAATAAAGTAATACTGCTTAGTTGTTGTGCCCTTAAAGCTTGATACATAAATGTATT AGTCCAGTCTCAATTATTTGGGAGCTAGCTGGGTGAAAGAAAACTGCAAAACGCTTATTAAAACGCCATT AACATCGGCTCTCAGAGGGTGGAGGCGACCTTCATGGTCTGACTGTGGAAAGAAAGAATGACAGCCACCT TCAGATTCAAAGCCTTTCTGGAGACCTGCAGCAGGCGGGTGCCAGCTCTAGCCACGGAAGGGGAGCCACC AAGATTAGGACGCACGGCAAGGTTGGCTTTACTTTGGAAGTTGTCAAAGGGGATGAACGGTAAAATAGTC TGGACAGAGGCTAAGTGTTCTATAAAATACTCTTAATTTTAAGAGGAGCCGAAGCTAGAGTTAAAATGGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 81633. Forward Primer - name:081633_F_cDNA_LOC434357, sequence:GAACAGGGAAGAAAATAGGGCT; Reverse Primer - name:081633_N_SP6_cDNA_LOC434357, sequence:TCCATTTTAACTCTAGCTTCGGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6261 same embryo
	EMAGE:6263 same embryo
	EMAGE:6260 same embryo
	EMAGE:6265 same embryo
	EMAGE:6266 same embryo
	EMAGE:6262 same embryo
	EurExpress:euxassay_016115 same experiment
	MGI:4822710 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source