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EMAGE:627

Pax3 paired box gene 3 ( MGI:97487)
TS14 (17 Somite no.)
in situ hybridisation

Data Images
EMAGE:627 EMAGE:627
17 somite embryo 17 somite embryo (Dorsal view)

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:627Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
627_wholemount_strong_3D_1.wlz
627_wholemount_moderate_3D_1.wlz
627_wholemount_weak_3D_1.wlz
627_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:627_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rhombomere 01
strong strong
regional
rhombomere 02
strong strong
Expression in dorsal r2 and neural crest cells adjacent to r2
rhombomere 04
strong strong
regionalExpression in dorsal r4 and neural crest cells adjacent to r4
head somite
strong strong
trunk somite
strong strong
caudal neuropore
not detected not detected
future midbrain
moderate moderate
regionalExpression level downregulated in the posterior midbrain when compared to r1
rhombomere 05
weak weak
Expression in r5 and r6 downregulated in comparison to r4
rhombomere 06
weak weak
Expression in r5 and r6 downregulated in comparison to r4
neural tube
strong strong
regionalExpressed in dorsal neural tube apart from the most posterior neurectoderm or posterior neuropore
prosencephalon
strong strong
regionalExpressed along dorsal midline
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1101193
Entity Detected:Pax3, paired box gene 3 ( MGI:97487)
Sequence:sense strand is shown

>MGI:1101193
AAGCTTACCGAGGCCCGAGTGCAGGTCTGGTTTAGCAACCGCCGTGCAAGATGGAGGAAACAAGCTGGAG
CCAATCAACTGATGGCTTTCAACCATCTCATTCCGGGGGGATTCCCTCCCACCGCCATGCCGACCCTGCC
AACATACCAGCTGTCGGAGACCTCTTACCAGCCCACGTCTATTCCACAAGCCGTGTCAGATCCCAGTAGC
ACCGTCCACAGACCTCAGCCGCTTCCTCCGAGCACTGTACACCAAAGCACTATTCCTTCGAACGCAGACA
GCAGCTCTGCCTACTGCCTCCCCAGCACCAGGCATGGATTTTCAAGCTATACAGACAGCTTTGTGCCTCC
ATCGGGGCCCTCCAACCCCATGAACCCCACCATCGGCAATGGCCTTTCACCTCAGGTAATGGGACTTCTG
ACCAACCACGGTGGGGTACCGCACCAGCCTCAGACCGACTATGCTCTCTCCCCTCTCACTGGGGGCCTGG
AACCCACGACCACGGTGTCAGCCAGCTGCA
nt 1157 - nt 1676 of NM_008781.2
Notes:Probe used in this study covers bases 516bases of the mouse Pax3 cDNA which corresponds to coding sequence and includes the last 67 bases of the homeobox. Probe originally described by Goulding et al., 1991 [PMID:2022185] as a Hind-PstI fragment.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:17 Somite no.
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Principal investigator:Ruth Arkell, MRC Mammalian Genetics Unit, null, Harwell, UK OX11 ORD
Submitted by:Ruth Arkell, MRC Mammalian Genetics Unit, null, Harwell, UK OX11 ORD
Experiment type:non-screen
References:[ PMID:2022185] Goulding MD, Chalepakis G, Deutsch U, Erselius JR, Gruss P 1991 Pax-3, a novel murine DNA binding protein expressed during early neurogenesis. EMBO J (10):1135-47
Links:MGI:1101193 same probe
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE