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EMAGE:6280

Rspo1 R-spondin homolog (Xenopus laevis) ( MGI:2183426)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6280 EMAGE:6280 EMAGE:6280 EMAGE:6280 EMAGE:6280
"Pseudo-wholemount" of euxassay_016147. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016147_01 euxassay_016147_02 euxassay_016147_03 euxassay_016147_04
EMAGE:6280 EMAGE:6280 EMAGE:6280 EMAGE:6280 EMAGE:6280
euxassay_016147_05 euxassay_016147_06 euxassay_016147_07 euxassay_016147_08 euxassay_016147_09
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euxassay_016147_10 euxassay_016147_11 euxassay_016147_12 euxassay_016147_13 euxassay_016147_14
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euxassay_016147_15 euxassay_016147_16 euxassay_016147_17 euxassay_016147_18 euxassay_016147_19
EMAGE:6280 EMAGE:6280 EMAGE:6280 EMAGE:6280 EMAGE:6280
euxassay_016147_20 euxassay_016147_21 euxassay_016147_22 euxassay_016147_23 euxassay_016147_24
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euxassay_016147_25 euxassay_016147_26 euxassay_016147_27

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6280Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6280_wholemount_strong.wlz
6280_wholemount_moderate.wlz
6280_wholemount_weak.wlz
6280_wholemount_possible.wlz
6280_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6280_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
telencephalon ventricular layer
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 14 15 16 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45158
Entity Detected:Rspo1, R-spondin homolog (Xenopus laevis) ( MGI:2183426)
Sequence:sense strand is shown

>T45158
GGTCTCCAGATACCTGTGGAAGAGTACAGTGCTGTACTGTATAATGAGAACTTTCCAGAACTGGAGCATC
TGGGAGAGTCCACACATACCCCATCCACCCACCCATCCAACTATCCATCCATCCATCCATGCACACATAT
GGCCACATCTGAAAACGTCAACACACACACACACACACACACACACACACACACACATTCTTGAGGTCAC
TGAAGACACTTCTATTCTGTGGCCCAGCTGTATATTCAGTCTTTAATGCTCTTGGAAGACATATCTGAGA
GAACCTTTCCCAGCATCTGAAACTAAGGAGTGGAACCTTCTGGAGGAACTTCTGGGACAGCATCTGACAG
ATGGATGGCAGATTGGAGCCAAAGCTGGAGCAGCTGCCGAGAGGGAGAGAGAGGGAAAGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 183829. Forward Primer - name:183829_F_exon_Rspondin, sequence:GGTCTCCAGATACCTGTGGAAG; Reverse Primer - name:183829_N_SP6_exon_Rspondin, sequence:GCTTTCCCTCTCTCTCCCTCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6278 same embryo
 EMAGE:6282 same embryo
 EMAGE:6279 same embryo
 EMAGE:6281 same embryo
 EMAGE:6277 same embryo
 EurExpress:euxassay_016147 same experiment
 MGI:4827825 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS