EMAGE mouse gene expression spatial database

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EMAGE:629

Bmp2 bone morphogenetic protein 2 ( MGI:88177)
TS10 (7.0 dpc)
in situ hybridisation

Data Images

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Anterior is to the left

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

629_wholemount_strong_3D_1.wlz

629_wholemount_moderate_3D_1.wlz

629_wholemount_weak_3D_1.wlz

629_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:629_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

allantois mesoderm

moderate

amniotic fold

moderate

visceral extraembryonic endoderm

strong

regional

Expressed in anterior visceral endoderm

yolk sac

moderate

embryo mesoderm

moderate

regional

Expressed in axial mesendoderm (ie. the mesoderm of midline structures (prechordal plate and notochord) as well as the definitive gut endoderm)

definitive endoderm

moderate

regional

Expressed in axial mesendoderm (ie. the definitive gut endoderm and mesoderm of midline structures (prechordal plate and notochord))

node

moderate

Annotation Validation:

submitter

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:11030
Entity Detected:	Bmp2, bone morphogenetic protein 2 ( MGI:88177)
Sequence:	sense strand is shown >MGI:11030 AACACCCGGAGAAGGAGGAGGAGGCGAAGAAAAGCAACAGAAGCCCAGTTGCTGCTCCAGGTCCCTCGGA CAGAGCTTTTTCCATGTGGAGACTCTCTCAATGGACGTGCCCCCTAGTGCTTCTTAGACGGACTGCGGTC TCCTAAAGCCGCAGGTCGACCATGGTGGCCGGGACCCGCTGTCTTCTAGTGTTGCTGCTTCCCCAGGTCC TCCTGGGCGGCGCGGCCGGCCTCATTCCAGAGCTGGGCCGCAAGAAGTTCGCCGCGGCATCCAGCCGACC CTTGTCCCGGCCTTCGGAAGACGTCCTCAGCGAATTTGAGTTGAGGCTGCTCAGCATGTTTGGCCTGAAG CAGAGACCCACCCCCAGCAAGGACGTCGTGGTGCCCCCCTATATGCTAGATCTGTACCGCAGGCACTCAG GCCAGCCAGGAGCGCCCGCCCCAGACCACCGGCTGGAGAGGGCAGCCAGCCGCGCCAACACCGTGCGCAG CTTCCATCAACTAGAAGCCGTGGAGGAACTTCCAGAGATGAGTGGGAAAACGGCCCGGCGCTTCTTCTTC AATTTAAGTTCTGTCCCCAGTGACGAGTTTCTCACATCTGCAGAACTCCAGATCTTCCGGGAACAGATAC AGGAAGCTTTGGGAAACAGTAGTTTCCAGCACCGAATTAATATTTATGAAATTATAAAGCCTGCAGCAGC CAACTTGAAATTTCCTGTGACCAGACTATTGGACACCAGGTTAGTGAATCAGAACACAAGTCAGTGGGAG AGCTTCGACGTCACCCCAGCTGTGATGCGGTGGACCACACAGGGACACACCAACCATGGGTTTGTGGTGG AAGTGGCCCATTTAGAGGAGAACCCAGGTGTCTCCAAGAGACATGTGAGGATTAGCAGGTCTTTGCACCA AGATGAACACAGCTGGTCACAGATAAGGCCATTGCTAGTGACTTTTGGACATGATGGAAAAGGACATCCG CTCCACAAACGAGAAAAGCGTCAA
	nt 197 - nt 1200 of XM_123927.1
Notes:	Probe used here corresponds to bases 197-1200 of the BMP2 cDNA (coding sequence) and was originally described by Lyons et al., 1989 [PMID:2481605] . Linearised with XmaIII, transcribed with Sp6.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	7.0 dpc
Theiler Stage:	TS10
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:	4% paraformaldehyde
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Principal investigator:	Ruth Arkell, MRC Mammalian Genetics Unit, null, Harwell, UK OX11 ORD
Submitted by:	Ruth Arkell, MRC Mammalian Genetics Unit, null, Harwell, UK OX11 ORD
Experiment type:	non-screen
References:	[ doi:10.1101/gad.3.11.1657] [ PMID:2481605] Lyons KM, Pelton RW, Hogan BL 1989 Patterns of expression of murine Vgr-1 and BMP-2a RNA suggest that transforming growth factor-beta-like genes coordinately regulate aspects of embryonic developm Genes Dev (3):1657-68
Links:	MGI:11030 same probe
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source