EMAGE mouse gene expression spatial database

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EMAGE:6294

AU021063 expressed sequence AU021063 ( MGI:2146136)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016185. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016185_01	euxassay_016185_02	euxassay_016185_03	euxassay_016185_04

euxassay_016185_05	euxassay_016185_06	euxassay_016185_07	euxassay_016185_08	euxassay_016185_09

euxassay_016185_10	euxassay_016185_11	euxassay_016185_12	euxassay_016185_13	euxassay_016185_14

euxassay_016185_15	euxassay_016185_16	euxassay_016185_17	euxassay_016185_18	euxassay_016185_19

euxassay_016185_20	euxassay_016185_21	euxassay_016185_22	euxassay_016185_23	euxassay_016185_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6294_wholemount_strong.wlz

6294_wholemount_moderate.wlz

6294_wholemount_weak.wlz

6294_wholemount_possible.wlz

6294_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6294_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

moderate

regional

moderate expression: see section 08 13 17 weak expression: see section 09 10 11 12 14 15 18

telencephalon

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 21 22 23 24 weak expression: see section 09 10 11 12 13 14 15 17 18 19 20

medulla oblongata

moderate

regional

moderate expression: see section 09 10 11 12 13 17 weak expression: see section 14 15 18 19 20

metencephalon

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 17 21 22 23 weak expression: see section 15 16 18 19 20

midbrain

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 17 21 weak expression: see section 15 16 18 19 20

facial vii ganglion

moderate

single cell

moderate expression: see section 06 21

glossopharyngeal ix ganglion

weak

single cell

weak expression: see section 08 19

trigeminal v ganglion

moderate

single cell

moderate expression: see section 04 05 06 07 17 18 21 22 weak expression: see section 08 09 19 20

vagus x ganglion

weak

single cell

weak expression: see section 09

spinal cord

moderate

regional

moderate expression: see section 12 13 14 15 16 17

dorsal root ganglion

moderate

single cell

moderate expression: see section 09 10 11 12 13 14 17 18 19 20

neural retina

weak

regional

weak expression: see section 01 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39817
Entity Detected:	AU021063, expressed sequence AU021063 ( MGI:2146136)
Sequence:	sense strand is shown >T39817 ACAAGACACCTGCTTCGACATAAGTTGCAGACGAAGTATAGATTTCTCAGGAGCAGATCCACAGGGGATT TGCTAAGAGGCATGAGGAGTGTGAACTCAGTTACCAGCTCACTACAGATCCTCCTGGAGGAACAAATGCT GTATGGGATCCCAAAATATGGCAAGGTTTCCAGGTGGCGCCGAGCACCAGACGGCTTTGAGTCTGGAGAT AGTTCCACCACAAGGCTCACGACCAGCAGAGGGTGCCACCATCCCACAGGGAGGCACCCAGGACTCTCCA ATCCTCCCTGGAGAAACTGTGGAACCGCAGAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 88106. Forward Primer - name:088106_F_cDNA_A330009N23Rik, sequence:ACAAGACACCTGCTTCGACATA; Reverse Primer - name:088106_N_SP6_cDNA_A330009N23Rik, sequence:CTCTGCGGTTCCACAGTTTCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6293 same assay
	EMAGE:6289 same embryo
	EMAGE:6295 same embryo
	EMAGE:6292 same embryo
	EMAGE:6290 same embryo
	EurExpress:euxassay_016185 same experiment
	MGI:4823352 same experiment
	MGI:4823351 same experiment
	EMAGE:6291 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source