EMAGE mouse gene expression spatial database

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EMAGE:6296

Txlnb taxilin beta ( MGI:2671945)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016227. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016227_01	euxassay_016227_02	euxassay_016227_03	euxassay_016227_04

euxassay_016227_05	euxassay_016227_06	euxassay_016227_07	euxassay_016227_08	euxassay_016227_09

euxassay_016227_10	euxassay_016227_11	euxassay_016227_12	euxassay_016227_13	euxassay_016227_14

euxassay_016227_15	euxassay_016227_16	euxassay_016227_17	euxassay_016227_18	euxassay_016227_19

euxassay_016227_20	euxassay_016227_21	euxassay_016227_22	euxassay_016227_23	euxassay_016227_24

euxassay_016227_25	euxassay_016227_26

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6296_wholemount_strong.wlz

6296_wholemount_moderate.wlz

6296_wholemount_weak.wlz

6296_wholemount_possible.wlz

6296_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6296_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

weak

single cell

weak expression: see section 10 11 17 18

glossopharyngeal ix ganglion

weak

single cell

weak expression: see section 09

trigeminal v ganglion

weak

single cell

weak expression: see section 06 07 08 09 10 21 22

ventral grey horn

weak

single cell

weak expression: see section 12 13 16 17

dorsal root ganglion

moderate

single cell

moderate expression: see section 09 10 11 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45430
Entity Detected:	Txlnb, taxilin beta ( MGI:2671945)
Sequence:	sense strand is shown >T45430 AGAGGCTTTTAACAGAAAGGGGAAAAAACACAAACAGGTGCTTCTGATCTTCTCAGCCAACTTCCTCCCA ACTTCCCAGCATCACCTCCTGGGTTGAGGCAGCCGCCTCCTGGCTACCTGTGAGAAGGTGGTTGGAAATA GAGAAAGCACCTAAGTAAGATTTGAGAGGTTGAGTATCCTCAGCTTAGAGGTCTCAGCCTCTTTGACTAC TGATATCTCAAGAAAACACTTTTTGTTGTCTCTGACATTGTCTTTATCTCAAAGCATCTATTCCTTCCAA ATTTCCTCCTTTGCGTCAATGTGTGTGCCTGGCCCACATCTCAATTAATGTTAAGTAAAATGTATTTTTC CAAAACGGAAATACATACGAGCTCTCAATAGGAGTAACAAGGCTTACAAAGCTAACACATTTTCTGCTCT AAGAATTTTTGAACAGGCAGAAGTACAAAATTTTTAAAAGGGGGGGGGGGCCTCCTACTCATAATTCAAA AATAACAGGCAAGGACTCCATAGATGTCAAAAGCAAGAAGCACTTGGGATTACCCAAACAAGTTTTATAC TCTCATTGTTCGCAAAAACCTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 183814. Forward Primer - name:183814_F_exon_2310001N14Rik, sequence:AGAGGCTTTTAACAGAAAGGGG; Reverse Primer - name:183814_N_SP6_exon_2310001N14Rik, sequence:GAGGTTTTTGCGAACAATGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6298 same embryo
	EMAGE:6297 same embryo
	EurExpress:euxassay_016227 same experiment
	MGI:4829030 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source