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EMAGE:63

Pax6 paired box gene 6 ( MGI:97490)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:63
Figure 3E of Suda et al., 2001 [PMID:11493561] . Copyright: This image is from Development and is displayed with permission from the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: this image has an asterisk marking the eye
Expression Pattern Description
Spatial Annotation:
EMAGE:63Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
63_wholemount_strong_3D_1.wlz
63_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:63_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
telencephalon
detected detected
diencephalon
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1277653
Entity Detected:Pax6, paired box gene 6 ( MGI:97490)
Sequence:sense strand is shown

>MGI:1277653
AATTCTGCAGACCCATGCAGATGCAAAAGTCCAGGTGCTGGACAATGAAAACGTATCCAACGGTTGTGTG
AGTAAAATTCTGGGCAGGTATTACGAGACTGGCTCCATCAGACCCAGGGCAATCGGAGGGAGTAAGCCAA
GAGTGGCGACTCCAGAAGTTGTAAGCAAAATAGCCCAGTATAAACGGGAGTGCCCTTCCATCTTTGCTTG
GGAAATCCGAGACAGATTATTATCCGAGGGGGTCTGTACCAACGATAACATACCCAGTGTGTCATCAATA
AACAGAGTTCTTCGCAACCTGG
nt 294 - nt 595 of X63963.1
Notes:Probe used in this study by Suda et al., 2001 [PMID:11493561] is indicated as being originally described by Walther & Gruss, 1991 [PMID:1687460] ie: "an EcoRI-NheI-cDNA-fragment encoding most of the 3' part of the Pax-6 paired box." Editors note: Restriction analysis of the sequence from Walther & Gruss (X63963.1) indicates it is likely to be the EcoRI-NheI fragment extending between nt294 and nt595 as the paired box domain extends between nt172 and nt597.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:undefined
General Information
Authors:Suda et al., 2001 [PMID:11493561] Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11493561] Suda Y, Hossain ZM, Kobayashi C, Hatano O, Yoshida M, Matsuo I, Aizawa S 2001 Emx2 directs the development of diencephalon in cooperation with Otx2. Development (128):2433-50
 [ PMID:1687460] Walther C, Gruss P 1991 Pax-6, a murine paired box gene, is expressed in the developing CNS. Development (113):1435-49
Links:MGI:2148277 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI